Rabbit anti-Human Homeodomain Interacting Protein Kinase 1 Polyclonal Antibody | anti-HIPK1 antibody

Homeodomain Interacting Protein Kinase 1 (HIPK1) Polyclonal Antibody

Cat. Num. AAA2032565

• Gene Names: HIPK1; Myak; Nbak2
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Homeodomain Interacting Protein Kinase 1; Polyclonal Antibody; Homeodomain Interacting Protein Kinase 1 (HIPK1) Polyclonal Antibody; Myak; Nbak2; Nuclear body-associated kinase 2; anti-HIPK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against HIPK1. It has been selected for its ability to recognize HIPK1 in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 1075

• Applicable Applications for anti-HIPK1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western Blot: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunoglobulin Type: IgG
• Conjugate: No Conjugate
• Cross Reactivity: Human
• Organism Species: Human
• Fragment: HIPK1 (Met1~Lys290)
• Quality Control: Content: The quality control contains recombinant HIPK1 (Met1~Lys290) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2066426
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded heart tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 38201638
• NCBI GeneID: 204851
• NCBI Accession #: NP_689909.2
• NCBI GenBank Nucleotide #: NM_152696.3
• UniProt Accession #: Q86Z02; O75125; Q5SQL2; Q5SQL4; Q5SQL5; Q8IYD7; Q8NDN5; Q8NEB6; Q8TBZ1; A6NJ34
• Molecular Weight: 87,302 Da
• NCBI Official Full Name: homeodomain-interacting protein kinase 1 isoform 2
• NCBI Official Synonym Full Names: homeodomain interacting protein kinase 1
• NCBI Official Symbol: HIPK1
• NCBI Official Synonym Symbols: Myak; Nbak2
• NCBI Protein Information: homeodomain-interacting protein kinase 1
• UniProt Protein Name: Homeodomain-interacting protein kinase 1
• UniProt Gene Name: HIPK1
• UniProt Synonym Gene Names: KIAA0630; MYAK; NBAK2

NCBI Description

The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

HIPK1: homeodomain interacting protein kinase 1 is a CMGC kinase of the DYRK family. May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Expression elevated in many breast cancer cell lines. Null mice are resistant to carcinogens. Binds the p53 tumor suppressor. 4 alternatively spliced isoforms of the human protein have been described.

Protein type: CMGC group; DYRK family; EC 2.7.11.1; HIPK subfamily; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: centrosome; cytoplasm; cytosol; nuclear speck; nucleoplasm; nucleus; PML body

Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity

Biological Process: anterior/posterior pattern specification; embryonic camera-type eye morphogenesis; embryonic retina morphogenesis in camera-type eye; endothelial cell apoptotic process; eye development; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; neuron differentiation; positive regulation of angiogenesis; positive regulation of cell proliferation; protein amino acid phosphorylation; regulation of transcription, DNA-templated; regulation of tumor necrosis factor-mediated signaling pathway; retina layer formation; smoothened signaling pathway; transcription, DNA-dependent