Rabbit anti-Mouse ND4L Polyclonal Antibody | anti-ND4L antibody

ND4L Polyclonal Antibody

Cat. Num. AAA3001428

• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ND4L; Polyclonal Antibody; ND4L Polyclonal Antibody; NADH-ubiquinone oxidoreductase chain 4L (EC: 1.6.5.3); NADH dehydrogenase subunit 4L; Mtnd4l; mt-Nd4l; Nd4l; anti-ND4L antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ND4L polyclonal antibody detects endogenous levels of ND4L protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 97

• Applicable Applications for anti-ND4L antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to amino acids 80-97 of Mouse ND4L.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ND4L polyclonal antibody at 1:1000 dilutionLane1:NIH/3T3 whole cell lysate (48ug))

Related Product Information for anti-ND4L antibody

NADH: ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 4L (ND4L) is most likely a component of the hydrophobic protein fragment of Complex I. Mutations in the gene encodiing for ND4 are implicated in Leber hereditary optic neuropathy, a rare condition that can cause loss of central vision.

NCBI and Uniprot Product Information

• NCBI GI #: 128890
• UniProt Accession #: P03903
• Molecular Weight: ~ 10kDa
• NCBI Official Full Name: NADH-ubiquinone oxidoreductase chain 4L
• UniProt Protein Name: NADH-ubiquinone oxidoreductase chain 4L
• UniProt Gene Name: Mtnd4l
• UniProt Synonym Gene Names: mt-Nd4l; Nd4l
• UniProt Entry Name: NU4LM_MOUSE

Uniprot Description

MT-ND4L: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in MT-ND4L are a cause of Leber hereditary optic neuropathy (LHON). LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes. Belongs to the complex I subunit 4L family.

Protein type: EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; Membrane protein, integral; Mitochondrial; Membrane protein, multi-pass