Rabbit anti-Human MT-ND3 Polyclonal Antibody | anti-MT-ND3 antibody

MT-ND3 Polyclonal Antibody

Cat. Num. AAA3006448

• Gene Names: MT-ND3; MTND3; ND3
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MT-ND3; Polyclonal Antibody; MT-ND3 Polyclonal Antibody; NADH-ubiquinone oxidoreductase chain 3; EC=1.6.5.3; NADH dehydrogenase subunit 3; MTND3; NADH3; ND3; anti-MT-ND3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MT-ND3 polyclonal antibody detects endogenous levels of MT-ND3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 115

• Applicable Applications for anti-MT-ND3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MT-ND3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MT-ND3 pAb at 1:500 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug))

Related Product Information for anti-MT-ND3 antibody

NADH: ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by trans-location of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 3 (ND3) localizes to the hydrophobic protein fragment of complex I. Mutations in the gene encodiing for ND3 may be associated with Parkinson disease.

NCBI and Uniprot Product Information

• NCBI GI #: 251831114
• NCBI GeneID: 4537
• NCBI Accession #: YP_003024033.1
• NCBI GenBank Nucleotide #: YP_003024033.1
• UniProt Accession #: P03897
• Molecular Weight: ~ 13kDa
• NCBI Official Full Name: NADH dehydrogenase subunit 3 (mitochondrion)
• NCBI Official Synonym Full Names: mitochondrially encoded NADH dehydrogenase 3
• NCBI Official Symbol: MT-ND3
• NCBI Official Synonym Symbols: MTND3; ND3
• NCBI Protein Information: NADH dehydrogenase, subunit 3 (complex I)
• UniProt Protein Name: NADH-ubiquinone oxidoreductase chain 3
• UniProt Gene Name: MT-ND3
• UniProt Synonym Gene Names: MTND3; NADH3; ND3
• UniProt Entry Name: NU3M_HUMAN

Uniprot Description

MT-ND3: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in MT-ND3 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. Defects in MT-ND3 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I subunit 3 family.

Protein type: EC 1.6.5.3; Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass; Mitochondrial; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: -