AGRP / AgRP recombinant protein

Recombinant Mouse AGRP / AgRP Protein (His tag)

Cat. Num. AAA2547061

• Gene Names: Agrp; Art; Agrt
• Purity: > 80 % as determined by SDS-PAGE
• Synonyms: AGRP / AgRP; Recombinant Mouse AGRP / AgRP Protein (His tag); Agrt; Art; AGRP / AgRP recombinant protein

• Host: Human Cells
• Purity/Purification: > 80 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 131

• Application Notes: The secreted recombinant mouse AGRP comprises 122 amino acids and has a calculated molecular mass of 13.7 kDa. The recombinant protein migrates as an approximately 20 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Val 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for AGRP / AgRP recombinant protein

Background: Agouti Related Protein (AGRP, or AGRT), is an endogenous antagonist of the melanocortin receptors MC3R and MC4R found in the hypothalamus and exhibits potent orexigenic activity. AGRP can act as a competitive antagonist to proopiomelanocortin (POMC)-derived peptides at the melanocortin-4 receptor (MC4R), and that this homeostatic mechanism is important as a means of coordinating appetite with perceived metabolic requirement. AGRP is upregulated by fasting while intracerebroventricular injections of synthetic AGRP lead to increased appetite and food intake. Thus, AGRP is a powerful orexigenic peptide that increases food intake when ubiquitously overexpressed or when administered centrally.

Description: A DNA sequence encoding the extracellular domain of mouse AGRP (P56473) (Met 1-Thr 131) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 424036681
• NCBI GeneID: 11604
• NCBI Accession #: NP_001258735.1
• NCBI GenBank Nucleotide #: NM_001271806.1
• UniProt Accession #: P56473; O35967
• Molecular Weight: 14,432 Da
• NCBI Official Full Name: agouti-related protein
• NCBI Official Synonym Full Names: agouti related neuropeptide
• NCBI Official Symbol: Agrp
• NCBI Official Synonym Symbols: Art; Agrt
• NCBI Protein Information: agouti-related protein
• UniProt Protein Name: Agouti-related protein
• UniProt Gene Name: Agrp
• UniProt Synonym Gene Names: Agrt; Art

NCBI Description

This gene encodes a protein that regulates feeding behavior and plays a key role in the control of body weight. The encoded protein acts as an antagonist of melanocortin receptor signaling. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

AGRP: Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. Genetic variations in AGRP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8|8 D3

Cellular Component: cell soma; extracellular space; Golgi lumen

Molecular Function: neuropeptide hormone activity

Biological Process: adult feeding behavior; eating behavior; neuropeptide signaling pathway

Disease: Obesity