Rabbit anti-Mouse ND6 Polyclonal Antibody | anti-ND6 antibody

ND6 Polyclonal Antibody

Cat. Num. AAA3001427

• Gene Names: mt-Nd6; ND6
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ND6; Polyclonal Antibody; ND6 Polyclonal Antibody; NADH-ubiquinone oxidoreductase chain 6 (EC: 1.6.5.3); NADH dehydrogenase subunit 6; Mtnd6; mt-Nd6; Nd6; anti-ND6 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ND6 polyclonal antibody detects endogenous levels of ND6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 172

• Applicable Applications for anti-ND6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to amino acids 72-86 of Mouse ND6.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ND6 polyclonal antibody at 1:1000 dilutionLane1:The muscle tissue lysate of Mouse (47ug))

Related Product Information for anti-ND6 antibody

NADH: ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. The significance of NADH dehydrogenase subunit 6 (ND6) is rapidly becoming increasingly apparent as many mutations leading to amino acid changes in this subunit are associated with known mitochondrial diseases.

NCBI and Uniprot Product Information

• NCBI GI #: 34538609
• NCBI GeneID: 17722
• NCBI Accession #: NP_904339.1
• NCBI GenBank Nucleotide #: NP_904339.1
• UniProt Accession #: P03925
• Molecular Weight: ~ 19kDa
• NCBI Official Full Name: NADH dehydrogenase subunit 6 (mitochondrion)
• NCBI Official Synonym Full Names: NADH dehydrogenase 6, mitochondrial
• NCBI Official Symbol: mt-Nd6
• NCBI Official Synonym Symbols: ND6
• NCBI Protein Information: NADH dehydrogenase subunit 6
• UniProt Protein Name: NADH-ubiquinone oxidoreductase chain 6
• UniProt Gene Name: Mtnd6
• UniProt Synonym Gene Names: mt-Nd6; Nd6
• UniProt Entry Name: NU6M_MOUSE

Uniprot Description

MT-ND6: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in MT-ND6 are a cause of Leber hereditary optic neuropathy (LHON). LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes. Defects in MT-ND6 are a cause of Leber hereditary optic neuropathy with dystonia (LDYT); also called familial dystonia with visual failure and striatal lucencies. LDYT is part of a spectrum of Leber hereditary optic neuropathy. It is characterized by the association of optic atrophy and central vision loss with dystonia. Defects in MT-ND6 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS). MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness. Defects in MT-ND6 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical manifestations from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I subunit 6 family.

Protein type: Membrane protein, integral; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; EC 1.6.5.3; Membrane protein, multi-pass

Cellular Component: mitochondrion; membrane; integral to membrane

Molecular Function: NADH dehydrogenase (ubiquinone) activity; oxidoreductase activity

Biological Process: transport