Western Blot (WB)
(Western blot analysis of extracts from PC-12 cells, untreated or TPA-treated, using MBS613016 Na,K-ATPase α1 Phosphorylated (Ser16) Antibody (upper) or total Na/K ATPase α1 Antibody MBS613016 (lower).)
Rabbit anti-Rat Na,K-ATPase alpha1 Polyclonal Antibody
Na,K-ATPase alpha1, phosphorylated (Ser16)
Reactivity
Rat
Applications
Western Blot
Purity
Purified using protein A and peptide affinity chromatography.
Synonyms
Na; K-ATPase alpha1; Polyclonal Antibody; phosphorylated (Ser16); Anti -Na; anti-Na; K-ATPase alpha1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of Na,KATPase 1 only when phosphorylated at Ser16.
Purity/Purification
Purified using protein A and peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Applicable Applications for anti-Na,K-ATPase alpha1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser16 of rat Na,K-ATPase alpha1.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from PC-12 cells, untreated or TPA-treated, using MBS613016 Na,K-ATPase α1 Phosphorylated (Ser16) Antibody (upper) or total Na/K ATPase α1 Antibody MBS613016 (lower).)
Western Blot (WB)
(Western blot analysis of extracts from PC-12 cells, untreated or TPA-treated, using MBS613016 Na,K-ATPase α1 Phosphorylated (Ser16) Antibody (upper) or total Na/K ATPase α1 Antibody MBS613016 (lower).)
Related Product Information for anti-Na,K-ATPase alpha1 antibody
The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic subunit and a subunit (reviewed in 1). Several phosphorylation sites have been identified for the 1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).
Product Categories/Family for anti-Na,K-ATPase alpha1 antibody
Similar Products
Product Notes
The Na,K-ATPase alpha1 (Catalog #AAA613016) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Na,K-ATPase alpha1, phosphorylated (Ser16) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Na,K-ATPase alpha1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the Na,K-ATPase alpha1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Na,K-ATPase alpha1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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