Rabbit ATP1A1 Polyclonal Antibody | anti-ATP1A1 antibody

ATP1A1 Polyclonal Antibody

Cat. Num. AAA8527342

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: ATP1A1; Polyclonal Antibody; ATP1A1 Polyclonal Antibody; MGC3285; MGC51750; Na; K-ATPase; anti-ATP1A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1023

• Applicable Applications for anti-ATP1A1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Fusion protein of human ATP1A1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of A549 cell and 293 cell line, using ATP1A1 antibody.)

Related Product Information for anti-ATP1A1 antibody

The Na, K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic alpha subunit and a beta subunit (reviewed in 1). Several phosphorylation sites have been identified for the alpha1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na, K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na, K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the alpha subunit (7). Na, K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8, 9).

References

1. Therien, A.G. and Blostein, R. (2000) Am. J. Physiol. Cell Physiol. 279, C541-566. 2. Féraille, E. et al. (1999) Mol. Biol. Cell 10, 2847-2859. 3. Fisone, G. et al. (1994) J. Biol. Chem. 269, 9368-9373. 4. Feschenko, M.S. and Sweadner, K.J. (1995) J. Biol. Chem. 270, 14072-14077. 5. Beguin, P. et al. (1994) J. Biol. Chem. 269, 24437-24445. 6. Yingst, D.R. et al. (2004) Am. J. Physiol. Renal Physiol. 287, F713-F721. 7. Al-Khalili, L. et al. (2004) J. Biol. Chem. 279, 25211-25218. 8. Tian, J. et al. (2006) Mol. Biol. Cell 17, 317-326. 9. Liang, M. et al. (2006) J. Biol. Chem. 281, 19709-19719.

NCBI and Uniprot Product Information

• NCBI GI #: 21361181
• NCBI GeneID: 476
• NCBI Accession #: NP_000692.2
• NCBI GenBank Nucleotide #: NM_000701.7
• UniProt Accession #: P05023; Q16689; Q6LDM4; Q9UCN1; Q9UJ20; Q9UJ21; B2RBR6; B7Z2T5; B7Z3U6; F5H3A1
• Molecular Weight: 56kDa
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1 isoform a
• NCBI Official Synonym Full Names: ATPase Na+/K+ transporting subunit alpha 1
• NCBI Official Symbol: ATP1A1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1A1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.