Rabbit AMP Activated Protein Kinase alpha1 Polyclonal Antibody | anti-KINB2 antibody

AMP Activated Protein Kinase alpha1, phosphorylated (Ser485)/AMPKa2 (Ser491) (AMPK a1, AMPK a2)

Cat. Num. AAA617100

• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography
• Synonyms: AMP Activated Protein Kinase alpha1; Polyclonal Antibody; phosphorylated (Ser485)/AMPKa2 (Ser491) (AMPK a1; AMPK a2); Anti -AMP Activated Protein Kinase alpha1; anti-KINB2 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of human AMPKa1/a2 only when phosphorylated at serine 485 or serine 491. This antibody does not cross-react with other related proteins. Species Crossreactivity: Mouse, monkey and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-KINB2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000. A band is detected at 62kD.
• Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser491 of human AMPKa2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from HEK293 and C6 cells, lamda phosphatase-treated or oligomycin-treated, using MBS617100 (upper) or A1475-04(lower).)

Related Product Information for anti-KINB2 antibody

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals andplays a key role in the regulation of energy homeostasis 1). AMPK is a heterotrimeric complex composed of a catalytic a subunit and regulatory b and g subunits, each of which is encoded by two or three distinct genes a1, 2; b1, 2; g1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia (1). Tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPK at Thr172 at the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKa is also phosphorylated at Thr258 and Ser485 for a1; Ser491 for a2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The b1 subunit is posttranslationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the b1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKg subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1).

Product Categories/Family for anti-KINB2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 6686784
• UniProt Accession #: Q9SCY5; O23481; F4JLS7
• Molecular Weight: 31,993 Da
• NCBI Official Full Name: AKIN beta2
• UniProt Protein Name: SNF1-related protein kinase regulatory subunit beta-2
• Protein Family: SNF1-related protein kinase regulatory
• UniProt Gene Name: KINB2
• UniProt Synonym Gene Names: AKIN subunit beta-2; AKINB2; AKINbeta2
• UniProt Entry Name: KINB2_ARATH

Uniprot Description

Function: Regulatory subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. The SnRK complex may also be involved in the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase and in assimilation of nitrogen by phosphorylating nitrate reductase.

Subunit structure: Subunit of a probable heterotrimeric complex consisting of an alpha catalytic (KIN10 or KIN11) subunit, and a beta (KINB) and a gamma (KING or SNF4) non-catalytic regulatory subunits. Interacts with SNF4. Ref.1 Ref.8 Ref.9

Tissue specificity: Expressed in leaves, stems, roots, flower buds and flowers. Not detectable in siliques. Ref.1

Induction: Repressed in the dark. Ref.1

Domain: Kinase-interacting sequence (KIS) is specific for the alpha catalytic subunit interaction and Association with SNF1 Complex (ASC) is specific for the gamma non-catalytic regulatory subunit interaction. Ref.7

Sequence similarities: Belongs to the 5'-AMP-activated protein kinase beta subunit family.

Sequence caution: The sequence AEE83735.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAB10413.1 differs from that shown. Reason: Frameshift at positions 11, 19 and 27. The sequence CAB78678.1 differs from that shown. Reason: Frameshift at positions 11, 19 and 27.