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Western Blot (WB)
(Western Blot analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein, using MBS609424)
Mouse ATPase alpha 1, Na, K Monoclonal Antibody
ATPase alpha 1, Na, K
Reactivity
Canine, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
AscitesAscities
Synonyms
ATPase alpha 1; Na; K; Monoclonal Antibody; Anti -ATPase alpha 1; anti-ATPase alpha 1; K antibody
Host
Mouse
Reactivity
Canine, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3C114
Specificity
Recognizes rabbit Na,K-ATPase alpha 1 subunit. Species Crossreactivity
Purity/Purification
Ascites
Ascities
Ascities
Form/Format
Supplied as a liquid, 0.1% sodium azide.
Applicable Applications for anti-ATPase alpha 1, Na, K antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Dilution: Western Blot: 1:1000-1:10,000 Detects a band at ~112kD. Do not boil the sample prior to loading on the gel (60 degree C is sufficient).
Immunohistochemistry (Paraffin): 1:200
Immunofluorescence: 1:100-1:1000
Dilution: Western Blot: 1:1000-1:10,000 Detects a band at ~112kD. Do not boil the sample prior to loading on the gel (60 degree C is sufficient).
Immunohistochemistry (Paraffin): 1:200
Immunofluorescence: 1:100-1:1000
Immunogen
Purified Na,K-ATPase from rabbit renal outer medulla
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein, using MBS609424)
Western Blot (WB)
(Western Blot analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein, using MBS609424)
Immunohistochemistry (IHC)
(Immunohistochemistry: Staining of enodmetrial glands within the uterus using MBS609424 Note the absence of staining in the surrounding myometrial smooth muscle.)
Immunohistochemistry (IHC)
(Immunohistochemistry: Staining of enodmetrial glands within the uterus using MBS609424 Note the absence of staining in the surrounding myometrial smooth muscle.)
Immunofluorescence (IF)
(mmunocytochemistry/Immunofluorescence of HepG2 cells using MBS609424.|Detection of ATPA1 (Green) Nuclei (Blue) were counterstained using Hoechst)
Immunofluorescence (IF)
(mmunocytochemistry/Immunofluorescence of HepG2 cells using MBS609424.|Detection of ATPA1 (Green) Nuclei (Blue) were counterstained using Hoechst)
Flow Cytometry (FC/FACS)
(IFlow Cytometry of fixed A549 cells using MBS609424.)
Flow Cytometry (FC/FACS)
(IFlow Cytometry of fixed A549 cells using MBS609424.)
Related Product Information for anti-ATPase alpha 1, Na, K antibody
Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, whereas the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Na,K-ATPase alpha-1 subunit, in brain, is expressed in both neuronal and non-neuronal cells.
Product Categories/Family for anti-ATPase alpha 1, Na, K antibody
Similar Products
Product Notes
The ATPase alpha 1, Na, K (Catalog #AAA609424) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATPase alpha 1, Na, K reacts with Canine, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's ATPase alpha 1, Na, K can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Dilution: Western Blot: 1:1000-1:10,000 Detects a band at ~112kD. Do not boil the sample prior to loading on the gel (60 degree C is sufficient). Immunohistochemistry (Paraffin): 1:200 Immunofluorescence: 1:100-1:1000. Researchers should empirically determine the suitability of the ATPase alpha 1, Na, K for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPase alpha 1, Na, K, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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