Testing Data
Rabbit N6-Methyladenosine Polyclonal Antibody
N6-Methyladenosine Antibody
Reactivity
Mammalian samples
Applications
Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation
Purity
Protein A and immunogen affinity purified antibody
Synonyms
N6-Methyladenosine; Polyclonal Antibody; N6-Methyladenosine Antibody; m6A; anti-N6-Methyladenosine antibody
Host
Rabbit
Reactivity
Mammalian samples
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A and immunogen affinity purified antibody
Form/Format
100 ug (1 mg/ml) in PBS (prepared using DEPC-treated water) with 0.09% (W/V) sodium azide.
Appearance: Colorless liquid
Appearance: Colorless liquid
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-N6-Methyladenosine antibody
Western Blot (WB), Dot Blot (DB), Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP)
Application Notes
Western blot: 1-4 ug/ml. Dot Blot, Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP).
Immunogen
This antibody is generated from rabbits immunized with a BSA conjugated N6-Methyladenosine (BV-N13-14).
Positive Control
Western blot: N6-Methyladenosine conjugated with BSA, Adenosine conjugated with BSA
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for anti-N6-Methyladenosine antibody
Polyclonal Antibody to detect N6-Methyladenosine in mammalian samples.
Background: N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse. The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.
Background: N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse. The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.
Product Categories/Family for anti-N6-Methyladenosine antibody
Similar Products
Product Notes
The N6-Methyladenosine (Catalog #AAA840935) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The N6-Methyladenosine Antibody reacts with Mammalian samples and may cross-react with other species as described in the data sheet. AAA Biotech's N6-Methyladenosine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Dot Blot (DB), Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP). Western blot: 1-4 ug/ml. Dot Blot, Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP). Researchers should empirically determine the suitability of the N6-Methyladenosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N6-Methyladenosine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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