Mouse Advanced Glycosylation End Product Specific Receptor ELISA Kit | AGER elisa kit

Mouse Advanced Glycosylation End Product Specific Receptor ELISA Kit

Cat. Num. AAA765159

• Gene Names: Ager; RAGE
• Reactivity: Mouse
• Synonyms: Advanced Glycosylation End Product Specific Receptor; Mouse Advanced Glycosylation End Product Specific Receptor ELISA Kit; RAGE/AGER/MOK protein kinase/MOKMAPK/MAK/MRK overlapping kinase/RAGE-1/renal tumor antigen/Renal tumor antigen 1; AGER elisa kit

• Reactivity: Mouse
• Sequence Length: 402

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 125-8000pg/ml
• Sensitivity: 75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AGER elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- RAGE antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- RAGE antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RAGE amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RAGE can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 259089426
• NCBI GeneID: 81722
• NCBI Accession #: NP_445788.2
• NCBI GenBank Nucleotide #: NM_053336.2
• Molecular Weight: 42,664 Da
• NCBI Official Full Name: advanced glycosylation end product-specific receptor
• NCBI Official Synonym Full Names: advanced glycosylation end product-specific receptor
• NCBI Official Symbol: Ager
• NCBI Official Synonym Symbols: RAGE
• NCBI Protein Information: advanced glycosylation end product-specific receptor
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• Protein Family: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: Ager
• UniProt Synonym Gene Names: Rage
• UniProt Entry Name: RAGE_RAT

NCBI Description

cell surface receptor for various molecules, including the amyloidogenic form of serum amyloid A [RGD, Feb 2006]

Uniprot Description

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Cell cycle regulation; Motility/polarity/chemotaxis; Membrane protein, integral

Cellular Component: axon; basal plasma membrane; cell soma; cell surface; cytoplasm; cytosol; extracellular region; extracellular space; integral to membrane; plasma membrane

Molecular Function: advanced glycation end-product receptor activity; heparin binding; identical protein binding; protein binding

Biological Process: activation of NF-kappaB transcription factor; astrocyte development; brain development; calcium ion homeostasis; glycoprotein metabolic process; induction of positive chemotaxis; inflammatory response; JAK-STAT cascade; lung development; negative regulation of cell adhesion; negative regulation of collagen biosynthetic process; negative regulation of endothelial cell proliferation; negative regulation of interleukin-10 production; negative regulation of osteoblast proliferation; negative regulation of protein amino acid phosphorylation; neurite development; positive regulation of activated T cell proliferation; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of cell migration; positive regulation of fibroblast proliferation; positive regulation of inflammatory response; positive regulation of interleukin-12 production; positive regulation of JNK activity; positive regulation of neuron apoptosis; positive regulation of phagocytosis, engulfment; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; regulation of DNA binding; regulation of inflammatory response; regulation of T cell mediated cytotoxicity; response to activity; response to fructose stimulus; response to genistein; response to hyperoxia; response to hypoxia; response to methylglyoxal; response to vitamin A