Human Progestagen Associated Endometrial Protein (PAEP) RTU ELISA Kit | PAEP rtu elisa kit

Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit

Cat. Num. AAA4501630

• Gene Names: PAEP; GD; GdA; GdF; GdS; PEP; PAEG; PP14; ZIF-1
• Reactivity: Human
• Synonyms: Progestagen Associated Endometrial Protein (PAEP); Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit; GD; GdA; GdF; GdS; PAEG; PEP; PP14; Glycodelin; Placental Protein 14; Alpha Uterine Protein; Pregnancy-Associated Endometrial Alpha-2-Globulin; Progesterone-associated; PAEP rtu elisa kit

• Reactivity: Human
• Sequence Length: 158

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 4.68-300ng/mL
• Sensitivity: 1.8ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAEP rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAEP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PAEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PAEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 937226996
• NCBI GeneID: 5047
• NCBI Accession #: NP_001018058.1
• NCBI GenBank Nucleotide #: NM_001018048.1
• UniProt Accession #: P09466; Q5T6T1; Q9UG92
• Molecular Weight: 9,953 Da
• NCBI Official Full Name: glycodelin isoform 2
• NCBI Official Synonym Full Names: progestagen associated endometrial protein
• NCBI Official Symbol: PAEP
• NCBI Official Synonym Symbols: GD; GdA; GdF; GdS; PEP; PAEG; PP14; ZIF-1
• NCBI Protein Information: glycodelin
• UniProt Protein Name: Glycodelin
• Protein Family: Glycodelin
• UniProt Gene Name: PAEP
• UniProt Synonym Gene Names: ZIF-1

NCBI Description

This gene is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. The encoded glycoprotein has been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestagen-associated endometrial protein. Three distinct forms, with identical protein backbones but different glycosylation profiles, are found in amniotic fluid, follicular fluid and seminal plasma of the reproductive system. These glycoproteins have distinct and essential roles in regulating a uterine environment suitable for pregnancy and in the timing and occurrence of the appropriate sequence of events in the fertilization process. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

Glycoprotein that regulates critical steps during fertilization and also has immunomonomodulatory effects. Four glycoforms, namely glycodelin-S, -A, -F and -C have been identified in reproductive tissues that differ in glycosylation and biological activity. Glycodelin-A has contraceptive and immunosuppressive activities (PubMed:9918684, PubMed:7531163). Glycodelin-C stimulates binding of spermatozoa to the zona pellucida (PubMed:17192260). Glycodelin-F inhibits spermatozoa-zona pellucida binding and significantly suppresses progesterone-induced acrosome reaction of spermatozoa (PubMed:12672671). Glycodelin-S in seminal plasma maintains the uncapacitated state of human spermatozoa (PubMed:15883155).