Immunohistochemistry (IHC)
(Immunochemical staining of rat MOG in rat brain with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Rat Myelin Oligodendrocyte Glycoprotein Polyclonal Antibody | anti-MOG antibody
Anti-Myelin Oligodendrocyte Glycoprotein Antibody, Rabbit Polyclonal
Reactivity
Rat
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Myelin Oligodendrocyte Glycoprotein; Polyclonal Antibody; Anti-Myelin Oligodendrocyte Glycoprotein Antibody; Rabbit Polyclonal; Myelin oligodendrocyte glycoprotein Antibody; Rabbit PAb; Antigen Affinity Purified; myelin oligodendrocyte glycoprotein; anti-MOG antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rat Myelin oligodendrocyte glycoprotein
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MOG antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
IHC-P: 1:10,000-1:50,000
ELISA: 1:25,000-1:50,000
IHC-P: 1:10,000-1:50,000
Immunogen
Recombinant Rat Myelin oligodendrocyte glycoprotein protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Rat Myelin oligodendrocyte glycoprotein (1PKQ_E; 1M-139H). Myelin oligodendrocyte glycoprotein specific IgG was purified by Rat Myelin oligodendrocyte glycoprotein affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of rat MOG in rat brain with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat MOG in rat brain with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Western Blot (WB)
(Anti-MOG rabbit polyclonal antibody at 1:500 dilution.Lane A: Rat brain tissue lysateLane B: Mouse brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:25 kDa)
Western Blot (WB)
(Anti-MOG rabbit polyclonal antibody at 1:500 dilution.Lane A: Rat brain tissue lysateLane B: Mouse brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:25 kDa)
Related Product Information for anti-MOG antibody
Myelin oligodendrocyte glycoprotein (MOG) is a transmembrane protein belonging to the immunoglobulin superfamily and contains an Ig-like domain followed by two potential membrane-spanning regions. MOG is expressed only in the CNS with very low content (approximately 0.1% total proteins) in the oligodendrogliocyte membrane. Three possible functions for MOG were suggested: (a) a cellular adhesive molecule, (b) a regulator of oligodendrocyte microtubule stability, and (c) a mediator of interactions between myelin and the immune system, in particular, the complement cascade. A direct interaction might exist between the membrane-associated regions of MOG and the myelin-specific glycolipid galactocerebroside (Gal-C), and such an interaction may have important consequences regarding the membrane topology and function of both molecules. It is considered that MOG is an autoantigen capable to produce demyelinating multiple sclerosis-like diseases in experimental animals.
References
Chekhonin VP, et al. (2003) Myelin oligodendrogliocyte glycoprotein: the structure, functions, role in pathogenesis of demyelinating disorders. Biomed Khim. 49(5): 411-23.Hilton AA, et al. (1995) Characterization of cDNA and Genomic Clones Encoding Human Myelin Oligodendrocyte Glycoprotein. J Neurochem. 65(1): 309-18.Johns TG, et al. (1999) The Structure and Function of Myelin Oligodendrocyte Glycoprotein. J Neurochem. 72(1): 1-9.
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Product Notes
The MOG (Catalog #AAA8113008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Myelin Oligodendrocyte Glycoprotein Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Myelin Oligodendrocyte Glycoprotein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IHC-P: 1:10,000-1:50,000. Researchers should empirically determine the suitability of the MOG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myelin Oligodendrocyte Glycoprotein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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