Rabbit anti-Human Bcr Monoclonal Antibody | anti-Bcr antibody

Bcr Rabbit mAb

Cat. Num. AAA4754138

• Gene Names: BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: Bcr; Monoclonal Antibody; Bcr Rabbit mAb; ALL; CML; PHL; BCR1; D22S11; D22S662; anti-Bcr antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 50nM Tris-Glycine(pH7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA

• Applicable Applications for anti-Bcr antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:2000; IHC: 1:20; ICC/IF: 1:50; FC/FACS: 1:20; IP: 1:20
• Immunogen: A synthetic peptide of human Bcr
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of Bcr in A549,HL-60,U2OS,C6 cell lysates using Bcr Rabbit mAb(1:1000 diluted).Predicted band size:143kDa.Observed band size:160kDa.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Bcr in paraffin-embedded Human Brain using Bcr Rabbit mAb at dilution 1/50)

Related Product Information for anti-Bcr antibody

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The unregulated tyrosine kinase activity of BCR-ABL1 contributes to the immortality of leukaemic cells. The BCR protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac and other kinases. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2020]

Product Categories/Family for anti-Bcr antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 82546843
• NCBI GeneID: 613
• NCBI Accession #: NP_004318.3
• NCBI GenBank Nucleotide #: NM_004327.3
• UniProt Accession #: P11274; P78501; Q12842; Q4LE80; Q6NZI3
• Molecular Weight: 142,819 Da
• NCBI Official Full Name: breakpoint cluster region protein isoform 1
• NCBI Official Synonym Full Names: breakpoint cluster region
• NCBI Official Symbol: BCR
• NCBI Official Synonym Symbols: ALL; CML; PHL; BCR1; D22S11; D22S662
• NCBI Protein Information: breakpoint cluster region protein; BCR/FGFR1 chimera protein; FGFR1/BCR chimera protein; renal carcinoma antigen NY-REN-26
• UniProt Protein Name: Breakpoint cluster region protein
• Protein Family: Bicyclomycin resistance protein
• UniProt Gene Name: BCR
• UniProt Synonym Gene Names: BCR1; D22S11
• UniProt Entry Name: BCR_HUMAN

NCBI Description

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Bcr: a protein with serine/threonine protein kinase activity and is a GTPase-activating protein (GAP) for RAC1 and CDC42. The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. A breakpoint cluster region protein that participates in a t(9;22)(q34;q11) chromosomal translocation that produces a BCR-ABL oncogene responsible for chronic myeloid leukemia (CML), acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator. Tyr177 of human Bcr, phosphorylated in the Bcr-Abl fusion protein, provides a docking site for Gab2 and GRB2, and is important in the transforming activity of Bcr-Abl. Sequence variants of the Bcr protein may be associated with bipolar disorder. Two alternatively spliced isoforms of the human protein have been reported.

Protein type: Kinase, protein; GAPs; GAPs, Rac/Rho; Oncoprotein; GEFs, Rac/Rho; Protein kinase, atypical; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; BCR family

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: postsynaptic membrane; protein complex; membrane; postsynaptic density; cytosol; cell junction

Molecular Function: protein serine/threonine kinase activity; Rho guanyl-nucleotide exchange factor activity; protein binding; enzyme binding; protein-tyrosine kinase activity; kinase activity; ATP binding; GTPase activator activity

Biological Process: inner ear morphogenesis; peptidyl-tyrosine phosphorylation; regulation of cell cycle; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; response to lipopolysaccharide; signal transduction; protein amino acid phosphorylation; negative regulation of neutrophil degranulation; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of phagocytosis; negative regulation of inflammatory response; brain development; actin cytoskeleton organization and biogenesis; neuromuscular process controlling balance; negative regulation of cell migration

Disease: Leukemia, Acute Lymphoblastic; Leukemia, Chronic Myeloid