Immunohistochemistry (IHC)
(Immunochemical staining of mouse GHR in mouse stomach with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Mouse Growth Hormone Receptor Polyclonal Antibody | anti-GHR antibody
Anti-Growth Hormone Receptor Antibody, Rabbit Polyclonal
Gene Names
Ghr; GHBP; GHR/BP
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Growth Hormone Receptor; Polyclonal Antibody; Anti-Growth Hormone Receptor Antibody; Rabbit Polyclonal; Growth Hormone Receptor/GHR/GHBP Antibody; Rabbit PAb; Antigen Affinity Purified; growth hormone receptor; Anti-GHBP Antibody; Anti-GHR/BP Antibody; anti-GHR antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Growth Hormone Receptor/GHR/GHBP
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GHR antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
IHC-P: 1:2000-1:10,000
ELISA: 1:25,000-1:50,000
IHC-P: 1:2000-1:10,000
Immunogen
Recombinant Mouse Growth Hormone Receptor/GHR/GHBP protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Growth Hormone Receptor/GHR/GHBP (rM Growth Hormone Receptor/GHR/GHBP). Growth Hormone Receptor/GHR/GHBP specific IgG was purified by Mouse Growth Hormone Receptor/GHR/GHBP affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse GHR in mouse stomach with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse GHR in mouse stomach with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse GHR in mouse kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse GHR in mouse kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Western Blot (WB)
(Anti-GHR rabbit polyclonal antibody at 1:500 dilutionLane A: PC-3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:71 kDaObserved band size:55 kDa)
Western Blot (WB)
(Anti-GHR rabbit polyclonal antibody at 1:500 dilutionLane A: PC-3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:71 kDaObserved band size:55 kDa)
Related Product Information for anti-GHR antibody
Growth hormone receptor, also known as GH receptor and GHR, is a single-pass type I membrane protein which belongs to thetype I cytokine receptor family and type 1 subfamily. GHR contains onefibronectin type-III domain. Growth hormone receptor/GHR is expressed in various tissues with high expression in liver and skeletal muscle. Isoform4of GHR is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform1 expression of GHR in placenta is predominant in chorion and decidua. Isoform4is highly expressed in placental villi. Isoform2of GHR is expressed in lung, stomach and muscle. Growth hormone receptor/GHR is a receptor for pituitary gland growth hormone. It is involved in regulating postnatal body growth. On ligand binding, it couples to the JAK2/STAT5 pathway. Isoform2of GHR up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. Defects in GHR are a cause of Laron syndrome (LARS) which is a severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may also be a cause of idiopathic short stature autosomal (ISSA) which is defined by a subnormal rate of growth.
References
Leung DW. et al., 1987, Nature. 330:537-43. Sobrier M-L. et al., 1997, J Clin Endocrinol Metab. 82: 435-7. Enberg B. et al., 2000, Eur J Endocrinol. 143: 71-6. Pantel J. et al., 2000, J Biol Chem. 275: 18664-9. Jorge AAL. et al., 2004, Clin Endocrinol. (Oxf.) 60: 36-40.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,105 Da
NCBI Official Full Name
growth hormone receptor isoform 1
NCBI Official Synonym Full Names
growth hormone receptor
NCBI Official Symbol
Ghr
NCBI Official Synonym Symbols
GHBP; GHR/BP
NCBI Protein Information
growth hormone receptor
Protein Family
Research Articles on GHR
Similar Products
Product Notes
The GHR (Catalog #AAA8110846) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Growth Hormone Receptor Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Growth Hormone Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IHC-P: 1:2000-1:10,000. Researchers should empirically determine the suitability of the GHR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Growth Hormone Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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