Western Blot (WB)
(MGMT Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).)
Rabbit anti-Human MGMT Polyclonal Antibody | anti-Mgmt antibody
MGMT, NT (MGMT, Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase)
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Flow Cytometry: 1:10-50
Western Blot (WB)
(MGMT Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).)
Western Blot (WB)
(MGMT Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).)
Flow Cytometry (FC/FACS)
(MGMT Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MGMT Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
enzyme involved in DNA repair of O(6)-alkylguanine which is the major mutagenic and carcinogenic lesion in DNA [RGD, Feb 2006]
Uniprot Description
MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.
Protein type: DNA repair, damage; EC 2.1.1.63; Apoptosis; Methyltransferase; Nuclear receptor co-regulator
Cellular Component: nucleoplasm; membrane; nucleus
Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding
Biological Process: response to drug; methylation; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA repair; regulation of caspase activity; response to organic cyclic substance; response to DNA damage stimulus
Research Articles on Mgmt
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Product Notes
The Mgmt mgmt (Catalog #AAA6005484) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGMT, NT (MGMT, Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGMT can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the Mgmt mgmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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