Western Blot (WB)
(MGMT Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).)
Rabbit anti-Human MGMT Polyclonal Antibody | anti-MGMT antibody
MGMT, NT (MGMT, Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase) (MaxLight 490)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
MGMT; Polyclonal Antibody; NT (MGMT; Methylated-DNA-protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase) (MaxLight 490); Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA-alkyltransferase; anti-MGMT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-MGMT antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MGMT.
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(MGMT Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).)
Western Blot (WB)
(MGMT Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).)
Flow Cytometry (FC/FACS)
(MGMT Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MGMT Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-MGMT antibody
MaxLight 490 is a new Blue-Green photostable dye conjugate comparable to DyLight488, Alexa Fluor488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Product Categories/Family for anti-MGMT antibody
References
Svrcek, M., et al. Gut 59(11):1516-1526(2010). Palli, D., et al. Mutagenesis 25(6):569-575(2010). Kim, M., et al. Cancer Sci. 101(11):2436-2442(2010). Mangoni, M., et al. Int. J. Radiat. Oncol. Biol. Phys. (2010). In press: Jacob, R., et al. Anticancer Res. 30(7):2561-2566(2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,646 Da
NCBI Official Full Name
Methylated-DNA--protein-cysteine methyltransferase
NCBI Official Synonym Full Names
O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol
MGMT
NCBI Protein Information
methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
UniProt Protein Name
Methylated-DNA--protein-cysteine methyltransferase
Protein Family
UniProt Gene Name
MGMT
UniProt Synonym Gene Names
MGMT
UniProt Entry Name
MGMT_HUMAN
Uniprot Description
MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.
Protein type: EC 2.1.1.63; Methyltransferase; Nuclear receptor co-regulator; DNA repair, damage; Apoptosis
Chromosomal Location of Human Ortholog: 10q26
Cellular Component: nucleoplasm; membrane; nucleus
Molecular Function: methyltransferase activity; DNA binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity
Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation
Research Articles on MGMT
Similar Products
Product Notes
The MGMT mgmt (Catalog #AAA6320674) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGMT, NT (MGMT, Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGMT can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MGMT mgmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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