Typical Testing Data/Standard Curve (for reference only)
Monkey Phospholipase A1 ELISA Kit | PLA1 elisa kit
Monkey Phospholipase A1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PLA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PLA1 antibody and an PLA1-HRP conjugate. The assay sample and buffer are incubated together with PLA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLA1 concentration since PLA1 from samples and PLA1-HRP conjugate compete for the anti-PLA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PLA1 from the sample, fewer sites are left to bind PLA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLA1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
Oct11: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen. Belongs to the POU transcription factor family. Class- 2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus
Molecular Function: protein dimerization activity; protein binding; sequence-specific DNA binding
Biological Process: keratinocyte differentiation; regulation of transcription from RNA polymerase II promoter; epidermis development; wound healing; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
Research Articles on PLA1
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Product Notes
The Monkey PLA1 pou2f3 (Catalog #AAA742899) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA742899 ELISA Kit recognizes Monkey PLA1. It is sometimes possible for the material contained within the vial of "Phospholipase A1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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