Rabbit POLB Polyclonal Antibody | anti-POLB antibody

POLB Antibody

Cat. Num. AAA9401533

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: POLB; Polyclonal Antibody; POLB Antibody; MGC125976; anti-POLB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total POLB protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 335

• Applicable Applications for anti-POLB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:200 - 1:1000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human POLB.
• Target Name: POLB
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using POLB antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human stomach cancer using POLB antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat lung using POLB antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using POLB antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-POLB antibody

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.

Product Categories/Family for anti-POLB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4505931
• NCBI GeneID: 5423
• NCBI Accession #: NP_002681.1
• NCBI GenBank Nucleotide #: NM_002690.2
• UniProt Accession #: P06746; Q3KP48; Q6FI34; B2RC78
• Molecular Weight: 38,178 Da
• NCBI Official Full Name: DNA polymerase beta
• NCBI Official Synonym Full Names: polymerase (DNA directed), beta
• NCBI Official Symbol: POLB
• NCBI Protein Information: DNA polymerase beta
• UniProt Protein Name: DNA polymerase beta
• Protein Family: DNA polymerase
• UniProt Gene Name: POLB
• UniProt Entry Name: DPOLB_HUMAN

NCBI Description

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]

Uniprot Description

POLB: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47. Belongs to the DNA polymerase type-X family.

Protein type: EC 2.7.7.7; DNA replication; DNA repair, damage; Lyase; Transferase

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: nucleoplasm; microtubule; spindle microtubule; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; microtubule binding; metal ion binding; lyase activity; DNA-directed DNA polymerase activity; damaged DNA binding

Biological Process: base-excision repair, gap-filling; neuron apoptosis; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; base-excision repair; pyrimidine dimer repair; nucleotide-excision repair, DNA gap filling; DNA repair; DNA-dependent DNA replication; response to DNA damage stimulus