Western Blot (WB)
(Western blot analysis of Factor D expression in mouse spleen extract (lane 1). Factor D at 43KD was detected using rabbit anti- Factor D Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
anti-Mouse Factor D Polyclonal Antibody | anti-Cfd antibody
Anti-Factor D Antibody
Gene Names
Cfd; DF; Adn
Reactivity
Mouse
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Factor D; Polyclonal Antibody; Anti-Factor D Antibody; Complement factor D; Adipsin; Adipsin/complement factor D; Adn; C3 convertase activator; CFAD_HUMAN; CFD; complement factor D preproprotein; D component of complement; DF; FactorD; PFD; Properdin factor D; complement factor D (adipsin); anti-Cfd antibody
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
258
Applicable Applications for anti-Cfd antibody
Western Blot (WB)
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunogen
E Coli-derived mouse Factor D recombinant protein (Position: Q30-K235). Mouse Factor D shares 66.5% and 87.4% amino acid (aa) sequence identity with human and rat Factor D, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Western blot analysis of Factor D expression in mouse spleen extract (lane 1). Factor D at 43KD was detected using rabbit anti- Factor D Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of Factor D expression in mouse spleen extract (lane 1). Factor D at 43KD was detected using rabbit anti- Factor D Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Related Product Information for anti-Cfd antibody
Description: Rabbit IgG polyclonal antibody for Complement factor D(CFD) detection. Tested with WB in Mouse.
Background: Factor D is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. It is also a serine protease that is secreted by adipocytes into the bloodstream. And it stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology.
Background: Factor D is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. It is also a serine protease that is secreted by adipocytes into the bloodstream. And it stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology.
References
1. Biesma, D. H., Hannema, A. J., van Velzen-Blad, H., Mulder, L., van Zwieten, R., Kluijt, I., Roos, D. A family with complement factor D deficiency. J. Clin. Invest. 108: 233-240, 2001. 2. Ronti T, Lupattelli G, Mannarino E (April 2006). "The endocrine function of adipose tissue: an update". Clin. Endocrinol. (Oxf) 64 (4): 355-65. 3. Volanakis JE, Narayana SV (April 1996). "Complement factor D, a novel serine protease". Protein Sci. 5 (4): 553-64.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,986 Da
NCBI Official Full Name
complement factor D isoform 2
NCBI Official Synonym Full Names
complement factor D (adipsin)
NCBI Official Symbol
Cfd
NCBI Official Synonym Symbols
DF; Adn
NCBI Protein Information
complement factor D
UniProt Protein Name
Complement factor D
Protein Family
UniProt Gene Name
Cfd
UniProt Synonym Gene Names
Adn; Df
UniProt Entry Name
CFAD_MOUSE
Uniprot Description
CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family.
Protein type: Protease; Secreted, signal peptide; EC 3.4.21.46; Secreted
Cellular Component: extracellular region; extracellular space
Molecular Function: endopeptidase activity; hydrolase activity; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity
Biological Process: complement activation, alternative pathway; immune system process; innate immune response; Notch signaling pathway; proteolysis
Research Articles on Cfd
Similar Products
Product Notes
The Cfd cfd (Catalog #AAA178237) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Factor D Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Factor D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the Cfd cfd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Factor D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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