Western Blot (WB)
(MIST Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MIST antibody detected the MIST protein (arrow).)
Rabbit anti-Human MIST Polyclonal Antibody | anti-MIST antibody
MIST, ID (CLNK, MIST, Cytokine-dependent hematopoietic cell linker, Mast cell immunoreceptor signal transducer) (Biotin)
Gene Names
CLNK; MIST
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MIST; Polyclonal Antibody; ID (CLNK; Cytokine-dependent hematopoietic cell linker; Mast cell immunoreceptor signal transducer) (Biotin); CLNK; Mast cell immunoreceptor signal transducer; anti-MIST antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-MIST antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
MIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-308 amino acids from the Central region of human MIST.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MIST Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MIST antibody detected the MIST protein (arrow).)
Western Blot (WB)
(MIST Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MIST antibody detected the MIST protein (arrow).)
Related Product Information for anti-MIST antibody
MIST is a member of the SLP76 family of adaptors (see LCP2, MIM 601603; BLNK, MIM 604515). MIST plays a role in the regulation of immunoreceptor signaling, including PLC-gamma (PLCG1; MIM 172420)-mediated B cell antigen receptor (BCR) signaling and FC-epsilon R1 (see FCER1A, MIM 147140)-mediated mast cell degranulation (Cao et al., 1999 [PubMed 10562326]; Goitsuka et al., 2000, 2001 [PubMed 10744659] [PubMed 11463797]).
Product Categories/Family for anti-MIST antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Cronin, S., et al. Eur. J. Hum. Genet. 17(2):213-218(2009). Goitsuka, R., et al. J. Biol. Chem. 276(38):36043-36050(2001). Goitsuka, R., et al. Int. Immunol. 12(4):573-580(2000). Cao, M.Y., et al. J. Exp. Med. 190(10):1527-1534(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,554 Da
NCBI Official Full Name
cytokine-dependent hematopoietic cell linker
NCBI Official Synonym Full Names
cytokine-dependent hematopoietic cell linker
NCBI Official Symbol
CLNK
NCBI Official Synonym Symbols
MIST
NCBI Protein Information
cytokine-dependent hematopoietic cell linker; mast cell immunoreceptor signal transducer
UniProt Protein Name
Cytokine-dependent hematopoietic cell linker
UniProt Gene Name
CLNK
UniProt Synonym Gene Names
MIST
UniProt Entry Name
CLNK_HUMAN
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Product Notes
The MIST clnk (Catalog #AAA6320901) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MIST, ID (CLNK, MIST, Cytokine-dependent hematopoietic cell linker, Mast cell immunoreceptor signal transducer) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MIST clnk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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