Western Blot (WB)
(Sample Western Blot: Representative western blot with Anti-MEK1/2 (1:1000) using 10 ug, 20 ug, and 40 ug of 293 cell lysate.)
Rabbit MEK1/2 Polyclonal Antibody | anti-MEK1/2 antibody
Anti-MEK1/2
Reactivity
Western blot human MEK1, MEK2MEK1and MEK2 from other species may also be detectable.
Applications
Western Blot
Purity
Affinity purified using Protein G
Synonyms
MEK1/2; Polyclonal Antibody; Anti-MEK1/2; anti-MEK1/2 antibody
Host
Rabbit
Reactivity
Western blot human MEK1, MEK2
MEK1and MEK2 from other species may also be detectable.
MEK1and MEK2 from other species may also be detectable.
Clonality
Polyclonal
Specificity
Recognizes the MEK1and MEK2 proteins
Purity/Purification
Affinity purified using Protein G
Form/Format
TBS, 50% glycerol
Applicable Applications for anti-MEK1/2 antibody
Western Blot (WB)
Application Notes
WB: 19562:20561
Immunogen
Synthesized peptide corresponding to human MEK1 (210-220 aa) protein
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Western Blot: Representative western blot with Anti-MEK1/2 (1:1000) using 10 ug, 20 ug, and 40 ug of 293 cell lysate.)
Western Blot (WB)
(Sample Western Blot: Representative western blot with Anti-MEK1/2 (1:1000) using 10 ug, 20 ug, and 40 ug of 293 cell lysate.)
Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation with Anti-MEK1/2 (1:1000) using 30ng of His-MEK1 and GST-MEK2 recombinant proteins.)
Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation with Anti-MEK1/2 (1:1000) using 30ng of His-MEK1 and GST-MEK2 recombinant proteins.)
Related Product Information for anti-MEK1/2 antibody
MEK1 is a member of the dual specificity protein kinase family that acts as a mitogen-activated protein kinase (MAPK) kinase. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra-and intracellular signals. As an essential component of MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (1). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative disease such as cancer (2).
Product Categories/Family for anti-MEK1/2 antibody
References
1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 9: 726-735, 1995.
2. Sebolt-Leopold, J S. et al: Blockade of the MAP kinase pathway suppresses growth of colon tumors in vivo. Nature Med. 5: 810-816, 1999.
2. Sebolt-Leopold, J S. et al: Blockade of the MAP kinase pathway suppresses growth of colon tumors in vivo. Nature Med. 5: 810-816, 1999.
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Product Notes
The MEK1/2 (Catalog #AAA516063) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MEK1/2 reacts with Western blot human MEK1, MEK2 MEK1and MEK2 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's MEK1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 19562:20561. Researchers should empirically determine the suitability of the MEK1/2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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