MEK1 recombinant protein

MEK1, Active

Cat. Num. AAA515536

• Gene Names: MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• Applications: Western Blot
• Synonyms: MEK1; Active; MEK1 recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: Full Length
• Sequence Length: 2603

• Applicable Applications for MEK1 recombinant protein: Kinase Assay, Western Blot (WB)
• Species: Human
• Tag: GST tag
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for MEK1 recombinant protein

Description: Recombinant full-length human MEK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: MEK1 is a member of the dual specificity protein kinase family that acts as a mitogen-activated protein kinase (MAPK) kinase. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra- and intracellular signals. As an essential component of MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (1). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative disease such as cancer (2).

Product Categories/Family for MEK1 recombinant protein

Enzymes; Active Kinases

References

1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 9: 726-735, 1995. 2. Sebolt-Leopold, J S. et al: Blockade of the MAP kinase pathway suppresses growth of colon tumors in vivo. Nature Med. 5: 810-816, 1999.

NCBI and Uniprot Product Information

• NCBI GI #: 169790828
• NCBI GeneID: 5604
• NCBI Accession #: NM_002755.3
• NCBI GenBank Nucleotide #: NM_002755
• UniProt Accession #: Q02750
• Molecular Weight: ~72 kDa
• NCBI Official Full Name: Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1), mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: MAP2K1
• NCBI Official Synonym Symbols: CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• Protein Family: Meiosis-specific serine/threonine-protein kinase
• UniProt Gene Name: MAP2K1
• UniProt Synonym Gene Names: MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Uniprot Description

MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: cytosol; early endosome; endoplasmic reticulum; focal adhesion; Golgi apparatus; late endosome; mitochondrion; nucleus; plasma membrane

Molecular Function: MAP kinase kinase activity; protein binding; protein C-terminus binding; protein kinase activity; protein N-terminus binding; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity

Biological Process: activation of MAPK activity; cell cycle arrest; cell motility; chemotaxis; MAPKKK cascade; negative regulation of cell proliferation; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of mitotic cell cycle; regulation of stress-activated MAPK cascade; signal transduction

Disease: Cardiofaciocutaneous Syndrome 3; Noonan Syndrome 1