Rat Apoptotic protease-activating factor 1 ELISA Kit | Apaf1 elisa kit

Rat Apoptotic protease-activating factor 1 ELISA Kit

Cat. Num. AAA762706

• Gene Names: APAF1; CED4; APAF-1
• Reactivity: Rat
• Synonyms: Apoptotic protease-activating factor 1; Rat Apoptotic protease-activating factor 1 ELISA Kit; Apaf1/APAF-1apoptotic protease activating factor 1/apoptotic peptidase activating factor/apoptotic peptidase activating factor 1/apoptotic protease activating factor/apoptotic protease-activating factor 1/CED4; Apaf1 elisa kit

• Reactivity: Rat
• Sequence Length: 1237

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Apaf1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Apaf1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Apaf1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Apaf1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat Apaf1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7108333
• NCBI GeneID: 317
• NCBI Accession #: NP_037361.1
• NCBI GenBank Nucleotide #: NM_013229.2
• Molecular Weight: 37,976 Da
• NCBI Official Full Name: apoptotic protease-activating factor 1 isoform a
• NCBI Official Synonym Full Names: apoptotic peptidase activating factor 1
• NCBI Official Symbol: APAF1
• NCBI Official Synonym Symbols: CED4; APAF-1
• NCBI Protein Information: apoptotic protease-activating factor 1
• UniProt Protein Name: Apoptotic protease-activating factor 1
• Protein Family: Apoptotic protease-activating factor
• UniProt Gene Name: APAF1
• UniProt Synonym Gene Names: KIAA0413; APAF-1
• UniProt Entry Name: APAF_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 12q23

Cellular Component: apoptosome; cytosol; nucleus

Molecular Function: ADP binding; ATP binding; caspase activator activity; heat shock protein binding; identical protein binding; nucleotide binding; protein binding

Biological Process: aging; apoptosis; caspase activation; caspase activation via cytochrome c; cell differentiation; forebrain development; nervous system development; neural tube closure; neuron apoptosis; positive regulation of apoptosis; programmed cell death; protein homooligomerization; regulation of apoptosis; response to hypoxia; response to nutrient