Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein diaphanous homolog 3 (DIAPH3) Standard Curve.)
Mouse Protein Diaphanous Homolog 3 (DIAPH3) ELISA Kit | DIAPH3 elisa kit
Mouse Protein Diaphanous Homolog 3 (DIAPH3) ELISA Kit
Gene Names
Diaph3; Dia2; Drf3; Diap3; p134MDia2; 4930417P13Rik
Reactivity
Mouse
Synonyms
Protein Diaphanous Homolog 3 (DIAPH3); Mouse Protein Diaphanous Homolog 3 (DIAPH3) ELISA Kit; DIAPH3; RP11-26P21.1; DKFZp434C0931; DKFZp686A13178; DRF3; FLJ34705; diap3; mDia2; diaphanous homolog 3; diaphanous-related formin 3; DIAPH3 elisa kit
Reactivity
Mouse
Specificity
Mouse Protein diaphanous homolog 3 (DIAPH3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DIAPH3. No significant cross-reactivity or interference between Mouse DIAPH3 and analogues was observed.
Sequence Length
1171
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein diaphanous homolog 3 (DIAPH3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein diaphanous homolog 3 (DIAPH3) Standard Curve.)
Related Product Information for DIAPH3 elisa kit
Description: This Mouse Protein diaphanous homolog 3 (DIAPH3) ELISA Kit employs a two-site sandwich ELISA to quantitate DIAPH3 in samples. An antibody specific for DIAPH3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DIAPH3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIAPH3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIAPH3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DIAPH3 encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in DIAPH3 are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for DIAPH3.
Background: DIAPH3 encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in DIAPH3 are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for DIAPH3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein diaphanous homolog 3
NCBI Official Synonym Full Names
diaphanous related formin 3
NCBI Official Symbol
Diaph3
NCBI Official Synonym Symbols
Dia2; Drf3; Diap3; p134MDia2; 4930417P13Rik
NCBI Protein Information
protein diaphanous homolog 3
UniProt Protein Name
Protein diaphanous homolog 3
Protein Family
UniProt Gene Name
Diaph3
UniProt Synonym Gene Names
Diap3; DRF3; mDIA2
UniProt Entry Name
DIAP3_MOUSE
Research Articles on DIAPH3
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Product Notes
The Mouse DIAPH3 diaph3 (Catalog #AAA9717889) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717889 ELISA Kit recognizes Mouse DIAPH3. It is sometimes possible for the material contained within the vial of "Protein Diaphanous Homolog 3 (DIAPH3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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