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Western Blot (WB) (Western blot analysis of PIGR Antibody (C-term) in HepG2(lane 1),K562(lane 2),HL-60(lane 3) cell line lysates (35ug/lane).PIGR (arrow) was detected using the purified Pab.)

Rabbit anti-Human PIGR Polyclonal Antibody | anti-PIGR antibody

PIGR, CT (Polymeric Immunoglobulin Receptor, Poly-Ig Receptor, FLJ22667, Hepatocellular Carcinoma-associated Protein TB6, MGC125361, MGC125362) (APC)

Reactivity
Human
Applications
Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
PIGR; Polyclonal Antibody; CT (Polymeric Immunoglobulin Receptor; Poly-Ig Receptor; FLJ22667; Hepatocellular Carcinoma-associated Protein TB6; MGC125361; MGC125362) (APC); anti-PIGR antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PIGR.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-PIGR antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human PIGR (KLH).
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PIGR Antibody (C-term) in HepG2(lane 1),K562(lane 2),HL-60(lane 3) cell line lysates (35ug/lane).PIGR (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of PIGR Antibody (C-term) in HepG2(lane 1),K562(lane 2),HL-60(lane 3) cell line lysates (35ug/lane).PIGR (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with PIGR Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma with PIGR Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(PIGR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (PIGR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PIGR Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of PIGR Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PIGR Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of PIGR Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Product Categories/Family for anti-PIGR antibody
References
1. Ewing, R.M., et.al., Mol. Syst. Biol. 3, 89 (2007). 2. Orzech, E., Cohen, S., et.al., J. Biol. Chem. 275(20), 15207-15219 (2000).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
83,284 Da
NCBI Official Full Name
polymeric immunoglobulin receptor
NCBI Official Synonym Full Names
polymeric immunoglobulin receptor
NCBI Official Symbol
PIGR
NCBI Protein Information
polymeric immunoglobulin receptor
UniProt Protein Name
Polymeric immunoglobulin receptor
UniProt Gene Name
PIGR
UniProt Synonym Gene Names
PIgR; Poly-Ig receptor

NCBI Description

This gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

Research Articles on PIGR

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Product Notes

The PIGR pigr (Catalog #AAA6493411) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIGR, CT (Polymeric Immunoglobulin Receptor, Poly-Ig Receptor, FLJ22667, Hepatocellular Carcinoma-associated Protein TB6, MGC125361, MGC125362) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIGR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-1:500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PIGR pigr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIGR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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