Western Blot (WB)
(WB: 20ug of untreated (lane 1) or trichostatin A-treated (lane 2) Cos-1 cell lysate was probed with MBS6002257.)
Rabbit Lysine, Acetylated Polyclonal Antibody
Lysine, Acetylated (Acetyl-Lysine)
Applications
Western Blot, Immunoprecipitation, Gel Super Shift Assay
Purity
SerumSerum
Synonyms
Lysine; Acetylated; Polyclonal Antibody; Acetylated (Acetyl-Lysine); Anti -Lysine; anti-Lysine; Acetylated antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes acetyl-lysine containing proteins including histones, from sodium butyrate or Trichostatin A treated cells, p53 acetylated in vitro by p300 or PCAF, and chemically acetylated BSA.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid containing 0.05% sodium azide and 30% glycerol.
Applicable Applications for anti-Lysine, Acetylated antibody
Western Blot (WB), Immunoprecipitation (IP), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Immunoblot Analysis: 1:1000-1:2000 dilution of this lot detected: acetylated histones in acid-extracted proteins from sodium butyrate (5mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A). Chemically acetylated BSA (10ng) in a dot blot assay. A 1:1000 dilution of the previous lot of the antibody detected: acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5uM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones. (Panel B). GST-p53 (100ng) acetylated in vitro with either recombinant p300 or PCAF. (Panel C)
Immunoprecipitation: 5ul of a previous lot immunoprecipitated in vitro acetylated recombinant p300.
Dilution: Immunoblot Analysis: 1:1000-1:2000 dilution of this lot detected: acetylated histones in acid-extracted proteins from sodium butyrate (5mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A). Chemically acetylated BSA (10ng) in a dot blot assay. A 1:1000 dilution of the previous lot of the antibody detected: acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5uM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones. (Panel B). GST-p53 (100ng) acetylated in vitro with either recombinant p300 or PCAF. (Panel C)
Immunoprecipitation: 5ul of a previous lot immunoprecipitated in vitro acetylated recombinant p300.
Immunogen
A mixture of chemically acetylated antigens
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(WB: 20ug of untreated (lane 1) or trichostatin A-treated (lane 2) Cos-1 cell lysate was probed with MBS6002257.)
Western Blot (WB)
(WB: 20ug of untreated (lane 1) or trichostatin A-treated (lane 2) Cos-1 cell lysate was probed with MBS6002257.)
Product Categories/Family for anti-Lysine, Acetylated antibody
Similar Products
Product Notes
The Lysine, Acetylated (Catalog #AAA6002257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Lysine, Acetylated can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Gel Shift Assay (GS/EMSA). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Immunoblot Analysis: 1:1000-1:2000 dilution of this lot detected: acetylated histones in acid-extracted proteins from sodium butyrate (5mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A). Chemically acetylated BSA (10ng) in a dot blot assay. A 1:1000 dilution of the previous lot of the antibody detected: acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5uM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones. (Panel B). GST-p53 (100ng) acetylated in vitro with either recombinant p300 or PCAF. (Panel C) Immunoprecipitation: 5ul of a previous lot immunoprecipitated in vitro acetylated recombinant p300. Researchers should empirically determine the suitability of the Lysine, Acetylated for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lysine, Acetylated, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
