Rabbit anti-Human ATXN1L Polyclonal Antibody | anti-ATXN1L antibody

ATXN1L, ID (ATXN1L, BOAT, BOAT1, Ataxin-1-like, Brother of ataxin-1) (AP)

Cat. Num. AAA6276799

• Gene Names: ATXN1L; BOAT; BOAT1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATXN1L; Polyclonal Antibody; ID (ATXN1L; BOAT; BOAT1; Ataxin-1-like; Brother of ataxin-1) (AP); Brother of ataxin-1; anti-ATXN1L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ATXN1L antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ATXN1L antibody is generated from rabbits immunized with a KLH conjugated synthetic between 384-411 amino acids selected from the Central region of human ATXN1L.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ATXN1L Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ATXN1L antibody detected the ATXN1L protein (arrow).)

Related Product Information for anti-ATXN1L antibody

ATXN1L can suppress the cytotoxicity of ATXN1 in spinocerebellar ataxia type 1 (SCA1) (By similarity).

Product Categories/Family for anti-ATXN1L antibody

Antibodies; Ataxin

References

Bowman, A.B., et al. Nat. Genet. 39(3):373-379(2007). Mizutani, A., et al. EMBO J. 24(18):3339-3351(2005). Venter, J.C., et al. Science 291(5507):1304-1351(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 212549561
• NCBI GeneID: 342371
• NCBI Accession #: NP_001131147.1
• NCBI GenBank Nucleotide #: NM_001137675.3
• UniProt Accession #: P0C7T5
• Molecular Weight: 73,306 Da
• NCBI Official Full Name: ataxin-1-like
• NCBI Official Synonym Full Names: ataxin 1-like
• NCBI Official Symbol: ATXN1L
• NCBI Official Synonym Symbols: BOAT; BOAT1
• NCBI Protein Information: ataxin-1-like; brother of ATXN1; brother of ataxin-1
• UniProt Protein Name: Ataxin-1-like
• Protein Family: Ataxin
• UniProt Gene Name: ATXN1L
• UniProt Synonym Gene Names: BOAT; BOAT1; Brother of ATXN1
• UniProt Entry Name: ATX1L_HUMAN

Uniprot Description

Function: Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Can suppress ATXN1 cytotoxicity in spinocerebellar ataxia type 1 (SCA1)

By similarity. Ref.5

Subunit structure: Homodimer. Interacts with CIC

By similarity. Interacts (via AXH domain) with NCOR2. Interacts with ATXN1. Directly interacts with RBPJ; this interaction is disrupted in the presence of Notch intracellular domain. Competes with ATXN1 for RBPJ-binding. Ref.2 Ref.5

Subcellular location: Nucleus. Cell projection › dendrite. Note: Forms nuclear foci. Colocalizes with NCOR2 and HDAC3. Distributed beyond the nucleus into the cell body and dendrites in Purkinje cells and in inferior olive cells. Ref.2

Tissue specificity: Expressed in cerebellum and cerebral cortex. Ref.2

Sequence similarities: Belongs to the ATXN1 family.Contains 1 AXH domain.