Testing Data
(Acid-extracted proteins from untreated (lane 1) or sodium butyrate treated (lane 2) HeLa cells probed with MBS6013338 (4ug/ml).)
Mouse anti-Human Lysine Monoclonal Antibody
Lysine, Acetylated (Acetyl-Lysine) (MaxLight 490)
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
Lysine; Monoclonal Antibody; Acetylated (Acetyl-Lysine) (MaxLight 490); anti-Lysine antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
4H349
Specificity
Recognizes acetylated lysine-containing proteins including histones. Of all the possible H3 and H4 acetylation sites, this antibody exhibits highest affinity for histone H4 acetylated on lysine 8 and histone H3 acetylated on lysine 14. Species Crossreactivity: human. Species Sequence Homology: rat and mouse
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-Lysine antibody
Immunoprecipitation (IP), Western Blot (WB)
Immunogen
Mixture of chemically acetylated antigens
Conjugate
MaxLight490
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Testing Data
(Acid-extracted proteins from untreated (lane 1) or sodium butyrate treated (lane 2) HeLa cells probed with MBS6013338 (4ug/ml).)
Testing Data
(Acid-extracted proteins from untreated (lane 1) or sodium butyrate treated (lane 2) HeLa cells probed with MBS6013338 (4ug/ml).)
Testing Data
(Representative lot data. Untreated and sodium butyrated treated HeLa lysate was resolved by electrophoresis, transferred to PVDF membrane and probed withMBS6013338. Proteins were visualized using a IgG (HRP)goat anti-mouse secondary antibody and a chemiluminescence detection system.)
Testing Data
(Representative lot data. Untreated and sodium butyrated treated HeLa lysate was resolved by electrophoresis, transferred to PVDF membrane and probed withMBS6013338. Proteins were visualized using a IgG (HRP)goat anti-mouse secondary antibody and a chemiluminescence detection system.)
Testing Data
(Proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS6013338 (4ug/ml). Panel A: RIPA lysates of untreated (Lane 1) and Trichostatin A treated (5um, 24 hours) Cos-1 cells (Lane 2). Panel B: In vitro acetylation of GST-p53 in the absence (Lane 1) or presence of recombinant p300 (Lane 2).)
Testing Data
(Proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS6013338 (4ug/ml). Panel A: RIPA lysates of untreated (Lane 1) and Trichostatin A treated (5um, 24 hours) Cos-1 cells (Lane 2). Panel B: In vitro acetylation of GST-p53 in the absence (Lane 1) or presence of recombinant p300 (Lane 2).)
Product Categories/Family for anti-Lysine antibody
References
1. Pennisi, E., Science (1997) 275: 155. 2. Imhof, A., et al., (1997) Current Biology 7: 689. 3. Munshi, N., et al., (1998) Mol. Cell. 2: 457. 4. Liu, L., et al., (1999) Mol. Cell. Biol. 19: 1202. 5. Cesena, Teresa, et al. (2007) J. Biol. Chem. 282: 956-967.
Similar Products
Product Notes
The Lysine (Catalog #AAA6245595) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Lysine, Acetylated (Acetyl-Lysine) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lysine can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the Lysine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lysine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
