Rabbit anti-Human LYAR Polyclonal Antibody | anti-LYAR antibody

LYAR Antibody

Cat. Num. AAA9605369

• Gene Names: LYAR; ZLYAR; ZC2HC2
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: LYAR; Polyclonal Antibody; LYAR Antibody; Cell growth regulating nucleolar protein; Cell growth-regulating nucleolar protein; Likely ortholog of mouse Ly1 antibody reactive clone; Ly1 antibody reactive; Ly1 antibody reactive homolog (mouse); Ly1 antibody reactive homolog; LYAR_HUMAN; ZC2HC2; ZLYAR; anti-LYAR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LYAR antibody detects endogenous levels of total LYAR
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 379

• Applicable Applications for anti-LYAR antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Nucleus > Nucleolus.
• Tissue Specificity: Predominantly expressed in testis.
• Predicted Cross Reactivity: Sheep
• Similarity: Sheep (82%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from LOVO cells using LYAR antibody.)

Immunofluorescene (IF)

(MBS9605369 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-LYAR antibody

Function: Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed:24495227). Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed:25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed:28686580). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed:25092918). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).
Subunit Structure: Interacts with PRMT5; this interaction is direct (PubMed:25092918). Interacts with GNL2 and RPL23A (PubMed:26203195). Interacts with nucleolin/NCL; this interaction is direct (By similarity).
Similarity: The N-terminal zinc-finger domains are required for the appropriate production of 28S rRNA and the formation of pre-60S particles.

NCBI and Uniprot Product Information

• NCBI GI #: 224591430
• NCBI GeneID: 55646
• NCBI Accession #: NP_001139197.1
• NCBI GenBank Nucleotide #: NM_001145725.1
• UniProt Accession #: Q9NX58; Q6FI78; Q9NYS1; D3DVS4
• Molecular Weight: Observed: 44 kD; Predicted: 44 kDa
• NCBI Official Full Name: cell growth-regulating nucleolar protein
• NCBI Official Synonym Full Names: Ly1 antibody reactive
• NCBI Official Symbol: LYAR
• NCBI Official Synonym Symbols: ZLYAR; ZC2HC2
• NCBI Protein Information: cell growth-regulating nucleolar protein
• UniProt Protein Name: Cell growth-regulating nucleolar protein
• Protein Family: Cell growth-regulating nucleolar protein
• UniProt Gene Name: LYAR

Uniprot Description

Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed:24495227). Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed:25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed:28686580). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed:25092918). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal ().