Rabbit HOXA5 Polyclonal Antibody | anti-HOXA5 antibody

HOXA5 Polyclonal Antibody

Cat. Num. AAA3007532

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HOXA5; Polyclonal Antibody; HOXA5 Polyclonal Antibody; Homeobox protein Hox-1C; Homeobox protein Hox-A5; HOX1C; anti-HOXA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HOXA5 polyclonal antibody detects endogenous levels of HOXA5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 270

• Applicable Applications for anti-HOXA5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human HOXA5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of HOXA5 polyclonal antibody at 1:500 dilutionLane1:COS-7 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:Panc1 whole cell lysate (40ug) Lane4:MEF whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug))

Related Product Information for anti-HOXA5 antibody

HoxA5 (previously identified as Hox-1.3) is a transcriptional regulator of multiple target genes, including p53 and the progesterone receptor. It is a potent transactivator of p53 and may affect the response of breast cancer cells to DNA damage. In primary breast carcinomas, loss of p53 expression is coupled with loss of HoxA5 expression, suggesting that the loss of HoxA5 expression is important in tumorigenesis. HoxA5 is dynamically expressed during gut development and organogenesis of the respiratory tract, and is continuously expressed from the neonatal period into adult stages in cerebellar Purkinje cells. Expression of HoxA5 is necessary for the region-specific differentiation of the endoderm and differentiation of the myeloid pathway. HoxA5 is also essential for correct specification of the cervical and upper thoracic region of the skeleton and for proper patterning of the embryo.

NCBI and Uniprot Product Information

• NCBI GI #: 24497517
• NCBI Accession #: NP_061975.2
• NCBI GenBank Nucleotide #: NP_061975.2
• UniProt Accession #: P20719
• Molecular Weight: ~ 29kDa
• NCBI Official Full Name: homeobox protein Hox-A5
• UniProt Protein Name: Homeobox protein Hox-A5
• Protein Family: Homeobox protein
• UniProt Gene Name: HOXA5
• UniProt Synonym Gene Names: HOX1C
• UniProt Entry Name: HXA5_HUMAN

Uniprot Description

HOXA5: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5'-CYYNATTA[TG]Y-3'. Belongs to the Antp homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: cell migration; negative regulation of erythrocyte differentiation; positive regulation of myeloid cell differentiation; transcription, DNA-dependent; positive regulation of apoptosis; multicellular organism growth; embryonic skeletal morphogenesis; anterior/posterior pattern formation; regulation of mammary gland epithelial cell proliferation; respiratory system process; negative regulation of angiogenesis; thyroid gland development; positive regulation of transcription from RNA polymerase II promoter; alveolus development