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Western Blot (WB)
(Western Blot analysis of various cells using LYAR Polyclonal Antibody diluted at 1?2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)
Rabbit anti-Human LYAR Polyclonal Antibody | anti-LYAR antibody
LYAR polyclonal antibody
Gene Names
LYAR; ZLYAR; ZC2HC2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
LYAR; Polyclonal Antibody; LYAR polyclonal antibody; PNAS-5; Cell growth-regulating nucleolar protein; anti-LYAR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
LYAR Polyclonal Antibody detects endogenous levels of LYAR protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applicable Applications for anti-LYAR antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Application Notes
WB: 1/500-1/2000
IHC: 1/100-1/300
ELISA: 1/10,000
IHC: 1/100-1/300
ELISA: 1/10,000
Immunogen
The antiserum was produced against synthesized peptide derived from human LYAR. AA range: 241-290
Preparation and Storage
Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of various cells using LYAR Polyclonal Antibody diluted at 1?2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)
Western Blot (WB)
(Western Blot analysis of various cells using LYAR Polyclonal Antibody diluted at 1?2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)
Western Blot (WB)
(Western Blot analysis of SH-SY5Y cells using LYAR Polyclonal Antibody diluted at 1?2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)
Western Blot (WB)
(Western Blot analysis of SH-SY5Y cells using LYAR Polyclonal Antibody diluted at 1?2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)
Western Blot (WB)
(Western blot analysis of lysates from Jurkat, HepG2, and LOVO cells, using LYAR Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from Jurkat, HepG2, and LOVO cells, using LYAR Antibody. The lane on the right is blocked with the synthesized peptide.)
WB4
(Western Blot (WB))
WB4
(Western Blot (WB))
Related Product Information for anti-LYAR antibody
Background: LYAR, also named as ZLYAR, is 379 amino acid protein, which contains 2 C2H2-type zinc fingers. LYAR is expressed at at high levels in immature spermatocytes, early embryos and in fetal liver and thymus,with low expression in kidney and spleen. It is suggested that LYAR participates in regulating the stability of C23, a protein that is critical for maintaining the self-renewal and differentiation of embryonic stem cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,615 Da
NCBI Official Full Name
cell growth-regulating nucleolar protein
NCBI Official Synonym Full Names
Ly1 antibody reactive
NCBI Official Symbol
LYAR
NCBI Official Synonym Symbols
ZLYAR; ZC2HC2
NCBI Protein Information
cell growth-regulating nucleolar protein; Ly1 antibody reactive homolog
UniProt Protein Name
Cell growth-regulating nucleolar protein
Protein Family
UniProt Gene Name
LYAR
UniProt Entry Name
LYAR_HUMAN
Uniprot Description
LYAR:
Protein type: Nucleolus; Unknown function
Chromosomal Location of Human Ortholog: 4p16.3
Cellular Component: nucleolus; nucleus
Molecular Function: protein binding; metal ion binding
Similar Products
Product Notes
The LYAR lyar (Catalog #AAA3002384) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LYAR polyclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LYAR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA). WB: 1/500-1/2000 IHC: 1/100-1/300 ELISA: 1/10,000. Researchers should empirically determine the suitability of the LYAR lyar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LYAR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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