Rabbit anti-Human LRRFIP1 Polyclonal Antibody | anti-LRRFIP1 antibody

LRRFIP1 Antibody

Cat. Num. AAA151272

• Gene Names: LRRFIP1; GCF2; TRIP; FLAP1; GCF-2; FLAP-1; HUFI-1; FLIIAP1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: LRRFIP1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: LRRFIP1; Polyclonal Antibody; LRRFIP1 Antibody; GCF2; TRIP; FLAP1; GCF-2; FLAP-1; HUFI-1; FLIIAP1; Leucine-rich repeat flightless-interacting protein 1; GC-binding factor 2; LRR FLII-interacting protein 1; leucine rich repeat (in FLII) interacting protein 1; anti-LRRFIP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LRRFIP1 antibody is predicted to not cross-react with other LRRFIP family members. Three isoforms of LRRFIP1 are known to exist; this antibody will only recognize the two longer isoforms.
• Purity/Purification: LRRFIP1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 808

• Applicable Applications for anti-LRRFIP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: LRRFIP1 antibody can be used for detection of LRRFIP1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: LRRFIP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human LRRFIP1.
• Buffer: LRRFIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: LRRFIP1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LRRFIP1 in human colon tissue lysate with LRRFIP1 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 20 μg/mL.)

Related Product Information for anti-LRRFIP1 antibody

LRRFIP1 Antibody: LRRFIP1, also known as GC-binding factor 2 (GCF2), is a 738 amino acid transcriptional repressor that mainly plays a cytoskeletal role. It is primarily localized in the cytoplasm and preferentially binds to GC-rich dsDNA, but will also bind directly to dsRNA as well. The RNA binding domain encompasses a lysine-rich motif. LRRFIP1 interacts with the mammalian Flightless I (Fli-I) and is a key component in the cytoskeletal regulation of platelet function. LRRFIP1 and the related protein LRRFIP2 may modulate canonical WNT signaling and mediate the IRF3-induced production of type I interferon via a beta-catenin-dependent pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 212276096
• NCBI GeneID: 9208
• NCBI Accession #: NP_001131022
• NCBI GenBank Nucleotide #: NM_001137552.1
• UniProt Accession #: Q32MZ4; O75766; O75799; Q32MZ5; Q53T49; Q6PKG2; Q9Y607; E9PGZ2
• Molecular Weight: 73,060 Da
• NCBI Official Full Name: leucine-rich repeat flightless-interacting protein 1 isoform 3
• NCBI Official Synonym Full Names: leucine rich repeat (in FLII) interacting protein 1
• NCBI Official Symbol: LRRFIP1
• NCBI Official Synonym Symbols: GCF2; TRIP; FLAP1; GCF-2; FLAP-1; HUFI-1; FLIIAP1
• NCBI Protein Information: leucine-rich repeat flightless-interacting protein 1; GC-binding factor 2; NEDD8-conjugating enzyme; TAR RNA-interacting protein; LRR FLII-interacting protein 1
• UniProt Protein Name: Leucine-rich repeat flightless-interacting protein 1
• Protein Family: Leucine-rich repeat flightless-interacting protein
• UniProt Gene Name: LRRFIP1
• UniProt Synonym Gene Names: GCF2; TRIP; LRR FLII-interacting protein 1
• UniProt Entry Name: LRRF1_HUMAN

Uniprot Description

LRRFIP1: Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding. Belongs to the LRRFIP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoskeleton; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; double-stranded RNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of interferon type I production; innate immune response; negative regulation of transcription, DNA-dependent