Rabbit anti-Human, Mouse LIM/homeobox Protein 1.2 Polyclonal Antibody | anti-LMX1B antibody

LIM/homeobox Protein 1.2 (LMX 1.2, LMX-1.2, LMX1.2, LIM Homeobox Transcription Factor 1-beta, LMX1B, LIM/homeobox Protein LMX1B, MGC138325, MGC142051, NPS1)

Cat. Num. AAA625238

• Gene Names: LMX1B; NPS1; LMX1.2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: LIM/homeobox Protein 1.2; Polyclonal Antibody; LIM/homeobox Protein 1.2 (LMX 1.2; LMX-1.2; LMX1.2; LIM Homeobox Transcription Factor 1-beta; LMX1B; LIM/homeobox Protein LMX1B; MGC138325; MGC142051; NPS1); Anti -LIM/homeobox Protein 1.2 (LMX 1.2; anti-LMX1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LMX1B. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-LMX1B antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the Center region of human LMX1B (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of LMX1B Antibody (Center) in mouse heart tissue lysates (35ug/lane). LMX1B (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with LMX1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of LMX1B (arrow) using rabbit polyclonal LMX1B Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LMX1B gene.)

Related Product Information for anti-LMX1B antibody

LMX1B is required for the normal development of the dopaminergic system. Loss of dopaminergic neurons is associated with one of the most prominent human neurological disorders, Parkinson's disease (PD). Dopaminergic neurons play an important role in the control of multiple brain functions including voluntary movement alongside an array of behavioral processes such as mood, reward, addiction, and stress. Lmx1b is one of several transcription factors involved in the generation of dopaminergic neurons in the brain.

Product Categories/Family for anti-LMX1B antibody

Antibodies; Abs to Transcription Factors; Homeobox (HOX)

NCBI and Uniprot Product Information

• NCBI GI #: 292494915
• NCBI GeneID: 4010
• NCBI Accession #: NP_001167618.1
• NCBI GenBank Nucleotide #: NM_001174147.1
• UniProt Accession #: O60663; O75463; Q5JU95; Q6ISC9; F8W7W6
• Molecular Weight: 44,917 Da
• NCBI Official Full Name: LIM homeobox transcription factor 1-beta isoform 2
• NCBI Official Synonym Full Names: LIM homeobox transcription factor 1, beta
• NCBI Official Symbol: LMX1B
• NCBI Official Synonym Symbols: NPS1; LMX1.2
• NCBI Protein Information: LIM homeobox transcription factor 1-beta; LMX-1.2; LIM/homeobox protein 1.2; LIM/homeobox protein LMX1B
• UniProt Protein Name: LIM homeobox transcription factor 1-beta
• Protein Family: LIM/homeobox protein
• UniProt Gene Name: LMX1B
• UniProt Synonym Gene Names: LMX-1.2
• UniProt Entry Name: LMX1B_HUMAN

NCBI Description

This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

LMX1B: Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels. Defects in LMX1B are the cause of nail-patella syndrome (NPS); also known as onychoosteodysplasia. NPS is a disease that cause abnormal skeletal patterning and renal dysplasia. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Cell development/differentiation; Transcription factor

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleus

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; transcription factor activity

Biological Process: collagen fibril organization; transcription, DNA-dependent; in utero embryonic development; multicellular organismal development; neuron migration; cerebellum morphogenesis; limb morphogenesis; neuron differentiation; cell proliferation; organ growth; dorsal/ventral pattern formation; regulation of transcription, DNA-dependent; midbrain development; positive regulation of transcription from RNA polymerase II promoter; central nervous system neuron development

Disease: Nail-patella Syndrome