Rabbit anti-Human ITIH2 Polyclonal Antibody | anti-ITIH2 antibody

ITIH2, CT (Inter-alpha-Trypsin Inhibitor Heavy Chain H2, Inter-alpha-Inhibitor Heavy Chain 2, ITI Heavy Chain H2, ITI-HC2, H2P, IGHEP2, Inter-alpha-Trypsin Inhibitor Complex Component II, Serum-derived Hyaluronan-associated Protein, SHAP)

Cat. Num. AAA625589

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: ITIH2; Polyclonal Antibody; CT (Inter-alpha-Trypsin Inhibitor Heavy Chain H2; Inter-alpha-Inhibitor Heavy Chain 2; ITI Heavy Chain H2; ITI-HC2; H2P; IGHEP2; Inter-alpha-Trypsin Inhibitor Complex Component II; Serum-derived Hyaluronan-associated Protein; SHAP); Anti -ITIH2; anti-ITIH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ITIH2.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.
• Concentration: 1.8mg/ml (varies by lot)

• Applicable Applications for anti-ITIH2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Recommended Dilutions:; Indirect Flow Cytometry: 1:10-1:50; Western Blot: 1:1000; Immunohistochemistry (FFPE): 1:50-1:100; Optimal dilutions to be determined by the researcher.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 858-886 in the C-terminal region of human ITIH2. Species Sequence Homology: porcine
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ITIH2 antibody (C-term) in HepG2 cell line lysates (35ug/lane). ITIH2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ITIH2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ITIH2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ITIH2 antibody

ITIH2 may act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.

Product Categories/Family for anti-ITIH2 antibody

Antibodies; Abs to Protease Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 148238273
• NCBI GeneID: 512164
• NCBI Accession #: NP_002207
• NCBI GenBank Nucleotide #: NM_001098016.2
• UniProt Accession #: P19823
• NCBI Official Full Name: inter-alpha-trypsin inhibitor heavy chain H2
• NCBI Official Synonym Full Names: inter-alpha-trypsin inhibitor heavy chain 2<
• NCBI Official Symbol: ITIH2
• NCBI Protein Information: inter-alpha-trypsin inhibitor heavy chain H2; inter-alpha (globulin) inhibitor H2; inter-alpha globulin inhibitor H2 polypeptide
• Protein Family: Inter-alpha-trypsin inhibitor