Rabbit anti-Human TLE1 Polyclonal Antibody | anti-TLE1 antibody

Anti-TLE1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101062

• Gene Names: TLE1; ESG; ESG1; GRG1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: TLE1; Polyclonal Antibody; Anti-TLE1 Antibody; Rabbit Polyclonal; TLE1 Antibody; Rabbit PAb; Antigen Affinity Purified; transducin-like enhancer of split 1 (E(sp1) homolog; Drosophila); Anti-ESG Antibody; Anti-ESG1 Antibody; Anti-GRG1 Antibody; anti-TLE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TLE1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TLE1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC-P: 1:2500-1:10,000; ICC/IF: 1:1500-1:50,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human TLE1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TLE1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-TLE1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:83 kDaObserved band size:78 kDa(We are unsure as to the identity of these extra bands.))

Immunohistochemistry (IHC)

(Immunochemical staining of human TLE1 in human breast carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human TLE1 in human colon carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of TLE1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human TLE1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)

Related Product Information for anti-TLE1 antibody

Groucho (Gro)/Transducin-like enhancer of split (TLE) family proteins act as co-repressors of many transcription factors, and are involved in key signaling pathways. TLE1 is an oncogenic transcriptional co-repressor that exerts its repressive effects through binding of transcription factors. The Transducin-like enhancer of split 1 (TLE1) corepressor protein is overexpressed in human lung tumors and is a putative lung-specific oncogene.

NCBI and Uniprot Product Information

• NCBI GI #: 21541824
• NCBI GeneID: 7088
• NCBI Accession #: NP_005068.2
• NCBI GenBank Nucleotide #: NM_005077.3
• UniProt Accession #: Q04724; Q5T3G4; Q969V9; A8K495
• Molecular Weight: 83,201 Da
• NCBI Official Full Name: transducin-like enhancer protein 1
• NCBI Official Synonym Full Names: transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
• NCBI Official Symbol: TLE1
• NCBI Official Synonym Symbols: ESG; ESG1; GRG1
• NCBI Protein Information: transducin-like enhancer protein 1; e(Sp1) homolog; enhancer of split groucho 1; enhancer of split groucho-like protein 1; transducin-like enhancer of split 1, homolog of Drosophila E(sp1)
• UniProt Protein Name: Transducin-like enhancer protein 1
• Protein Family: Transducin-like enhancer protein
• UniProt Gene Name: TLE1
• UniProt Synonym Gene Names: ESG1
• UniProt Entry Name: TLE1_HUMAN

Uniprot Description

Function: Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG. Ref.13

Subunit structure: Homooligomer and heterooligomer with other family members. Binds LEF1, RUNX1, RUNX3, FOXA2, KDM6A, UTY, histone H3, HESX1, ESRRG and the NF-kappa-B subunit RELA. Interacts with HES1 (via WRPW motif). Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.16

Subcellular location: Nucleus. Note: Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components. Ref.15

Tissue specificity: In all tissues examined, mostly in brain, liver and muscle.

Post-translational modification: Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1. Ref.15Ubiquitinated by XIAP/BIRC4. Ref.21

Sequence similarities: Belongs to the WD repeat Groucho/TLE family.Contains 6 WD repeats.