Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit | PAEP elisa kit

Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit

Cat. Num. AAA2020606

• Gene Names: PAEP; GD; GdA; GdF; GdS; PEP; PAEG; PP14
• Reactivity: Human
• Synonyms: Progestagen Associated Endometrial Protein (PAEP); Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit; GD; GdA; GdF; GdS; PAEG; PEP; PP14; Glycodelin; Placental Protein 14; Alpha Uterine Protein; Pregnancy-Associated Endometrial Alpha-2-Globulin; Progesterone-associated; PAEP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Progestagen Associated Endometrial Protein (PAEP).; No significant cross-reactivity or interference between Progestagen Associated Endometrial Protein (PAEP) and analogues was observed.
• Sequence Length: 180

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 4.69-300ng/mL
• Sensitivity: < 2.1ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Progestagen Associated Endometrial Protein (PAEP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Progestagen Associated Endometrial Protein (PAEP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for PAEP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Progestagen Associated Endometrial Protein (PAEP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Progestagen Associated Endometrial Protein (PAEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Progestagen Associated Endometrial Protein (PAEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Progestagen Associated Endometrial Protein (PAEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PAEP elisa kit

Reproductive science

NCBI and Uniprot Product Information

• NCBI GI #: 109731808
• NCBI GeneID: 5047
• UniProt Accession #: P09466; Q5T6T1; Q9UG92
• Molecular Weight: 9,953 Da
• NCBI Official Full Name: Progestagen-associated endometrial protein
• NCBI Official Synonym Full Names: progestagen-associated endometrial protein
• NCBI Official Symbol: PAEP
• NCBI Official Synonym Symbols: GD; GdA; GdF; GdS; PEP; PAEG; PP14
• NCBI Protein Information: glycodelin
• UniProt Protein Name: Glycodelin
• Protein Family: Glycodelin
• UniProt Gene Name: PAEP
• UniProt Synonym Gene Names: GD; PP14; PAEG; PEG
• UniProt Entry Name: PAEP_HUMAN

NCBI Description

This gene is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. The encoded glycoprotein has been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestagen-associated endometrial protein. Three distinct forms, with identical protein backbones but different glycosylation profiles, are found in amniotic fluid, follicular fluid and seminal plasma of the reproductive system. These glycoproteins have distinct and essential roles in regulating a uterine environment suitable for pregnancy and in the timing and occurrence of the appropriate sequence of events in the fertilization process. A number of alternatively spliced transcript variants have been observed at this locus, but the full-length nature of only two, each encoding the same protein, has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PAEP: This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. Belongs to the calycin superfamily. Lipocalin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular region

Biological Process: transport; multicellular organismal development