Testing Data
(MBS611662 staining of mouse neural tube and limb bud respectively.)
Rabbit anti-Mouse Large Proteoglycan, GAG alpha domain Polyclonal Antibody
Large Proteoglycan, GAG alpha domain (Versican)
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Large Proteoglycan; GAG alpha domain; Polyclonal Antibody; GAG alpha domain (Versican); Anti -Large Proteoglycan; anti-Large Proteoglycan; GAG alpha domain antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse versican GAG alpha domain.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS.
Applicable Applications for anti-Large Proteoglycan, GAG alpha domain antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Immunohistochemistry, Western Blot and Immunoprecipitation.
Dilution: Immunohistochemistry (Formalin fixed, paraffin embedded): 10ug/ml. Tissues must be treated with Chondroitinase (PBS + 0.5U/ml of Chondroitinase) for 30 min. at RT prior to blocking and staining.
Western Blot: 0.5ug/ml.
Dilution: Immunohistochemistry (Formalin fixed, paraffin embedded): 10ug/ml. Tissues must be treated with Chondroitinase (PBS + 0.5U/ml of Chondroitinase) for 30 min. at RT prior to blocking and staining.
Western Blot: 0.5ug/ml.
Immunogen
GST fusion protein containing aa535-598 of mouse versican.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(MBS611662 staining of mouse neural tube and limb bud respectively.)
Testing Data
(MBS611662 staining of mouse neural tube and limb bud respectively.)
Related Product Information for anti-Large Proteoglycan, GAG alpha domain antibody
Versican is a large multi-domain chondroitin sulfate proteoglycan. It is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. Versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan. Versican has an apparent Mr of ~1000kD, of which the core contributes 400kD from the 2409 amino acids. The 12-15 covalently attached chondroitin sulfate chains make up the rest.
Product Categories/Family for anti-Large Proteoglycan, GAG alpha domain antibody
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Product Notes
The Large Proteoglycan, GAG alpha domain (Catalog #AAA611662) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Large Proteoglycan, GAG alpha domain (Versican) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Large Proteoglycan, GAG alpha domain can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in ELISA, Immunohistochemistry, Western Blot and Immunoprecipitation. Dilution: Immunohistochemistry (Formalin fixed, paraffin embedded): 10ug/ml. Tissues must be treated with Chondroitinase (PBS + 0.5U/ml of Chondroitinase) for 30 min. at RT prior to blocking and staining. Western Blot: 0.5ug/ml. Researchers should empirically determine the suitability of the Large Proteoglycan, GAG alpha domain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Large Proteoglycan, GAG alpha domain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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