Immunohistochemistry (IHC)
(Immunohistochemistry-HAPLN4 in Human Brain.)
Goat anti-Human HAPLN4 Polyclonal Antibody | anti-HAPLN4 antibody
HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4, Brain Link Protein 2)
Gene Names
HAPLN4; BRAL2; KIAA1926
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry
Purity
Affinity PurifiedPurified by human HAPLN4 affinity chromatography.
Synonyms
HAPLN4; Polyclonal Antibody; HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4; Brain Link Protein 2); Anti -HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4; anti-HAPLN4 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Selected for its ability to recognize human HAPLN4 in the applications listed. In Western Blots, this antibody shows less than 1% crossreactivity with rhHAPLN1.
Purity/Purification
Affinity Purified
Purified by human HAPLN4 affinity chromatography.
Purified by human HAPLN4 affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Applicable Applications for anti-HAPLN4 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human HAPLN4. The detection limit for rhHAPLN4 is approximately 10 ng/lane under non-reducing and reducing conditions., Immunoprecipitation: This antibody has been used to immunoprecipitate rhHAPLN4 from conditioned media of transfected NS0 cells., Immunocytochemistry: This antibody has been used at a concentration of 10ug/ml to detect HAPLN4 on SHSY5Y human neuroblastoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4 degree C followed by NorthernLightsTM 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA., Optimal dilutions should be deterhined by each laboratory for each application.
Immunogen
NS0-derived rhHAPLN4
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Immunohistochemistry-HAPLN4 in Human Brain.)
Immunohistochemistry (IHC)
(Immunohistochemistry-HAPLN4 in Human Brain.)
Related Product Information for anti-HAPLN4 antibody
HAPLN4 (hyaluronan and proteoglycan link protein 4; also Bral2) is a 40-42kD secreted member of the Link module superfamily. It is secreted by select neurons, particularly of the cerebellum, where it participates in ECM stability. Here it may link hyaluronic acid with resident proteoglycans. Mature HAPLN4 is 383 amino acids (aa) in length. It contains one lg-like C2-type domain (aa 46-161) and two link domains (aa 163-365). One potential alternate start site exists 36 aa upstream of the standard start site. Human and mouse HAPLN4 share 91% aa sequence identity.
Product Categories/Family for anti-HAPLN4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,801 Da
NCBI Official Full Name
hyaluronan and proteoglycan link protein 4
NCBI Official Synonym Full Names
hyaluronan and proteoglycan link protein 4
NCBI Official Symbol
HAPLN4
NCBI Official Synonym Symbols
BRAL2; KIAA1926
NCBI Protein Information
hyaluronan and proteoglycan link protein 4; brain link protein 2
UniProt Protein Name
Hyaluronan and proteoglycan link protein 4
Protein Family
UniProt Gene Name
HAPLN4
UniProt Synonym Gene Names
BRAL2; KIAA1926
UniProt Entry Name
HPLN4_HUMAN
Uniprot Description
HAPLN4: Binds to hyaluronic acid and may be involved in formation of the extracellular matrix. Belongs to the HAPLN family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19p13.1
Cellular Component: proteinaceous extracellular matrix
Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding
Biological Process: central nervous system development; cell adhesion; skeletal development
Research Articles on HAPLN4
Similar Products
Product Notes
The HAPLN4 hapln4 (Catalog #AAA618914) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4, Brain Link Protein 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HAPLN4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human HAPLN4. The detection limit for rhHAPLN4 is approximately 10 ng/lane under non-reducing and reducing conditions., Immunoprecipitation: This antibody has been used to immunoprecipitate rhHAPLN4 from conditioned media of transfected NS0 cells., Immunocytochemistry: This antibody has been used at a concentration of 10ug/ml to detect HAPLN4 on SHSY5Y human neuroblastoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4 degree C followed by NorthernLightsTM 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA., Optimal dilutions should be deterhined by each laboratory for each application. Researchers should empirically determine the suitability of the HAPLN4 hapln4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAPLN4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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