SDS-Page
(Reducing SDS-PAGE of 6ug of (MBS651750) pretreated with chondroitinase (Lane 1) and without chondroitinase|(Lane 2). Major bands representing proteoglycan|core proteins are visible at 400kD and 250kD. Lower|weight bands correspond to chondroitinase.)
Chondroitin Sulfate, Proteoglycan Mixture Native Protein
Chondroitin Sulfate, Proteoglycan Mixture (CSPG)
Purity
95% by PAGE analysis; Migrates as a large HMW (< 400kD) mass under reduced conditions. Mixture of large, extracellular chondroitin sulfate proteoglycans purified from embryonic chicken brains by chromatrographic methods. Following chondroitinase treatment
Synonyms
Chondroitin Sulfate; Proteoglycan Mixture; Proteoglycan Mixture (CSPG); Proteoglycan Mixture native protein
Host
Isolated from whole 12-14 day old embryonic chicken brains.
Purity/Purification
95% by PAGE analysis; Migrates as a large HMW (< 400kD) mass under reduced conditions. Mixture of large, extracellular chondroitin sulfate proteoglycans purified from embryonic chicken brains by chromatrographic methods. Following chondroitinase treatment
Form/Format
Supplied as a liquid in PBS. No preservatives added.
Concentration
0.75mg/ml (varies by lot)
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Reducing SDS-PAGE of 6ug of (MBS651750) pretreated with chondroitinase (Lane 1) and without chondroitinase|(Lane 2). Major bands representing proteoglycan|core proteins are visible at 400kD and 250kD. Lower|weight bands correspond to chondroitinase.)
SDS-Page
(Reducing SDS-PAGE of 6ug of (MBS651750) pretreated with chondroitinase (Lane 1) and without chondroitinase|(Lane 2). Major bands representing proteoglycan|core proteins are visible at 400kD and 250kD. Lower|weight bands correspond to chondroitinase.)
Related Product Information for Chondroitin Sulfate, Proteoglycan Mixture native protein
100ug protein as determined using the bicinchoninic acid (BCA) assay. Note that 100ug is a measurement of core polypeptides. Components of this mixture contain core polypeptide plus attached glycosaminoglycans. Contains a mixture of large, extracellular chondroitin sulfate proteoglycans isolated from embryonic chicken brain. Amino acid sequence analyses and immunological studies have demonstrated that major components of mixture are neurocan, phosphacan, versican and aggrecan.
Aggrecan, versican and neurocan are members of the family of hyaluronic acid-binding proteoglycans and therefore each contains hyaluronic acid binding regions, Ig-like domains, EGF like domains, lectin-like domains, and complement regulatory-like domains. Phosphacan is distinct; contains a region of similarity to carbonic anhydrase, a fibronectin type III domain, and is a splice variant of transmembrane protein receptor tyrosine phosphatase equivalent to its extracellular portion.
Although aggrecan was traditionally thought of as cartilage proteoglycan and neurocan and phosphacan were thought of as neural proteoglycans, recent studies indicate that all four proteoglycans have broad yet distinctive distribution patterns in early embryos. Presence of these proteoglycans has not been carefully examined in adult tissue beyond the expected sites. These proteoglycans are anti-adhesion molecules and capable of inhibiting cell-ECM and cell-cell adhesion.
Aggrecan, versican and neurocan are members of the family of hyaluronic acid-binding proteoglycans and therefore each contains hyaluronic acid binding regions, Ig-like domains, EGF like domains, lectin-like domains, and complement regulatory-like domains. Phosphacan is distinct; contains a region of similarity to carbonic anhydrase, a fibronectin type III domain, and is a splice variant of transmembrane protein receptor tyrosine phosphatase equivalent to its extracellular portion.
Although aggrecan was traditionally thought of as cartilage proteoglycan and neurocan and phosphacan were thought of as neural proteoglycans, recent studies indicate that all four proteoglycans have broad yet distinctive distribution patterns in early embryos. Presence of these proteoglycans has not been carefully examined in adult tissue beyond the expected sites. These proteoglycans are anti-adhesion molecules and capable of inhibiting cell-ECM and cell-cell adhesion.
Product Categories/Family for Chondroitin Sulfate, Proteoglycan Mixture native protein
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Product Notes
The Chondroitin Sulfate, Proteoglycan Mixture (Catalog #AAA651750) is a Native Protein produced from Isolated from whole 12-14 day old embryonic chicken brains. and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Chondroitin Sulfate, Proteoglycan Mixture, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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