Rabbit anti-Human, Mouse SIGLEC7 Polyclonal Antibody | anti-SIGLEC7 antibody

SIGLEC7, NT (Sialic Acid-binding Ig-like Lectin 7, Siglec-7, QA79 Membrane Protein, Adhesion Inhibitory Receptor Molecule-1, AIRM-1, p75, D-siglec, AIRM1, D-siglec, CDw328, CD328)

Cat. Num. AAA625378

• Gene Names: SIGLEC7; p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; p75/AIRM1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: SIGLEC7; Polyclonal Antibody; NT (Sialic Acid-binding Ig-like Lectin 7; Siglec-7; QA79 Membrane Protein; Adhesion Inhibitory Receptor Molecule-1; AIRM-1; p75; D-siglec; AIRM1; CDw328; CD328); Anti -SIGLEC7; anti-SIGLEC7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SIGLEC7. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-SIGLEC7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human D-SIGLEC (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-SIGLEC7 Pab in NCI-H460 cell line lysate (35ug/lane). SIGLEC7(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-SIGLEC7 antibody

SIGLECs are cell surface proteins of the Ig superfamily. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. A large subgroup of SIGLECs share high homology with SIGLEC3 (CD33) and are localized to 19q13.4. The cDNA for the SLG gene encodes 2 variants, SLG-long (SLGL) and SLG-short (SLGS). The 595aa SLGL protein contains a signal peptide and 2 V-set N-terminal Ig-like domains. The 477aa SLGS protein has a weak signal sequence and, like most SIGLEC3-like SIGLECs, has only 1 V-set N-terminal Ig-like domain. Both variants contain 2 C2-set N-terminal Ig-like domains, a transmembrane domain, and a cytoplasmic tail with a putative ITIM and a putative SLAM-like tyrosine-based motif. The conserved arginine residue thought to be essential for sialic acid binding in other SIGLECs is replaced by a glutamine in SLGS and by a cysteine in SLGL. RT-PCR analysis detected high expression of both variants in spleen and small intestine, and SLGS was highly expressed in adrenal gland and SLGL was highly expressed in bone marrow.

Product Categories/Family for anti-SIGLEC7 antibody

Antibodies; Abs to Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 7657570
• NCBI GeneID: 27036
• NCBI Accession #: NP_055200.1
• NCBI GenBank Nucleotide #: NM_014385.2
• UniProt Accession #: Q9Y286; Q9NZQ1; Q9UJ86; Q9UJ87; Q9Y502
• Molecular Weight: 51,143 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 7 isoform 1
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 7
• NCBI Official Symbol: SIGLEC7
• NCBI Official Synonym Symbols: p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; p75/AIRM1
• NCBI Protein Information: sialic acid-binding Ig-like lectin 7; AIRM-1; QA79 membrane protein; sialic acid binding immunoglobulin-like lectin 7; adhesion inhibitory receptor molecule 1, siglec-7
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 7
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC7
• UniProt Synonym Gene Names: AIRM1
• UniProt Entry Name: SIGL7_HUMAN

Uniprot Description

SIGLEC7: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane

Molecular Function: receptor activity; carbohydrate binding

Biological Process: cell adhesion