Western Blot (WB)
(KDM1B / LSD2 antibody (pAb) tested by Western blot. Nuclear extract of NIH3T3 cells (40 ug) probed with KDM1B / LSD2 antibody (pAb) at a dilution of 1:500.)
Rabbit anti-Mouse KDM1B/LSD2 Polyclonal Antibody | anti-KDM1B/LSD2 antibody
KDM1B/LSD2 antibody (pAb)
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity Purified
Synonyms
KDM1B/LSD2; Polyclonal Antibody; KDM1B/LSD2 antibody (pAb); anti-KDM1B/LSD2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-KDM1B/LSD2 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This antibody was raised against a peptide within the N-terminal region of mouse KDM1B/LSD2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(KDM1B / LSD2 antibody (pAb) tested by Western blot. Nuclear extract of NIH3T3 cells (40 ug) probed with KDM1B / LSD2 antibody (pAb) at a dilution of 1:500.)
Western Blot (WB)
(KDM1B / LSD2 antibody (pAb) tested by Western blot. Nuclear extract of NIH3T3 cells (40 ug) probed with KDM1B / LSD2 antibody (pAb) at a dilution of 1:500.)
Related Product Information for anti-KDM1B/LSD2 antibody
Short Description: Our KDM1B/LSD2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Mouse samples.
Background: KDM1B/LSD2 (Lysine (K)-Specific Demethylase 1B/Lysine-Specific Histone Demethylase 2) is a histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity).
Background: KDM1B/LSD2 (Lysine (K)-Specific Demethylase 1B/Lysine-Specific Histone Demethylase 2) is a histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity).
Product Categories/Family for anti-KDM1B/LSD2 antibody
NCBI and Uniprot Product Information
Molecular Weight
93kDa
Similar Products
Product Notes
The KDM1B/LSD2 (Catalog #AAA388905) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KDM1B/LSD2 antibody (pAb) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KDM1B/LSD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the KDM1B/LSD2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDM1B/LSD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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