Rabbit anti-Human REST Polyclonal Antibody | anti-REST antibody

REST antibody (pAb)

Cat. Num. AAA388957

• Gene Names: REST; WT6; XBR; HGF5; NRSF; DFNA27; GINGF5
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: REST; Polyclonal Antibody; REST antibody (pAb); XBR; NRSF; anti-REST antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

• Applicable Applications for anti-REST antibody: Western Blot (WB)
• Application Notes: Applications Validated:; WB: 1:500-1:1,000 dilution; Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
• Immunogen: This antibody was raised against a peptide within the C-terminal region of human REST.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(REST antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (40 ug) probed with REST antibody (1:500).)

Related Product Information for anti-REST antibody

Short Description: Our REST antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.

Background: REST (RE1-silencing transcription factor) is a transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

Product Categories/Family for anti-REST antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 301897974
• NCBI GeneID: 5978
• NCBI Accession #: NP_005603; AB_2793681
• NCBI GenBank Nucleotide #: NM_005612.5
• UniProt Accession #: Q13127
• Molecular Weight: 120kDa
• NCBI Official Full Name: RE1-silencing transcription factor
• NCBI Official Synonym Full Names: RE1 silencing transcription factor
• NCBI Official Symbol: REST
• NCBI Official Synonym Symbols: WT6; XBR; HGF5; NRSF; DFNA27; GINGF5
• NCBI Protein Information: RE1-silencing transcription factor
• UniProt Protein Name: RE1-silencing transcription factor
• Protein Family: Restin
• UniProt Gene Name: REST
• UniProt Synonym Gene Names: NRSF; XBR
• UniProt Entry Name: REST_HUMAN

NCBI Description

This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018]