Western Blot (WB)
(SETD7 / SET7 (pAb) tested by Western blot. Detection of SETD7 / SET7 by Western blot analysis. Nuclear extract of MCF-7 cells (20 ug) was probed with SETD7 / SET7 (pAb) at a 1:500 dilution.)
Rabbit anti-Human SETD7/SET7 Polyclonal Antibody | anti-SETD7/SET7 antibody
SETD7/SET7 antibody (pAb)
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Western Blot (WB)
(SETD7 / SET7 (pAb) tested by Western blot. Detection of SETD7 / SET7 by Western blot analysis. Nuclear extract of MCF-7 cells (20 ug) was probed with SETD7 / SET7 (pAb) at a 1:500 dilution.)
Western Blot (WB)
(SETD7 / SET7 (pAb) tested by Western blot. Detection of SETD7 / SET7 by Western blot analysis. Nuclear extract of MCF-7 cells (20 ug) was probed with SETD7 / SET7 (pAb) at a 1:500 dilution.)
Background: SETD7/SET7 (SET Domain Containing (Lysine Methyltransferase) 7) is a histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
NCBI and Uniprot Product Information
Uniprot Description
SETD7: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Interacts with IPF1/PDX-1. Widely expressed. Expressed in pancreatic islets. Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase
Chromosomal Location of Human Ortholog: 4q28
Cellular Component: nucleoplasm; nucleolus; chromosome
Molecular Function: protein binding; p53 binding; protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity
Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; chromatin modification; response to DNA damage stimulus
Research Articles on SETD7/SET7
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Product Notes
The SETD7/SET7 setd7 (Catalog #AAA388902) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SETD7/SET7 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SETD7/SET7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the SETD7/SET7 setd7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SETD7/SET7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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