Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lamina-associated polypeptide 2, isoform alpha (TMPO) Standard Curve.)
Mouse Lamina-Associated Polypeptide 2, Isoform alpha (TMPO) ELISA Kit | LAP2 elisa kit
Mouse Lamina-Associated Polypeptide 2, Isoform alpha (TMPO) ELISA Kit
Gene Names
Tmpo; TP; LAP2; AI195756; AI606875; AW214352; AW547477; 5630400D24Rik
Reactivity
Mouse
Synonyms
Lamina-Associated Polypeptide 2; Isoform alpha (TMPO); Mouse Lamina-Associated Polypeptide 2; Isoform alpha (TMPO) ELISA Kit; TMPO; CMD1T; LEMD4; MGC61508; PRO0868; TP; LEM domain containing 4; lamina-associated polypeptide 2; LAP2 elisa kit
Reactivity
Mouse
Specificity
Mouse Lamina-associated polypeptide 2, isoform alpha (TMPO) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMPO. No significant cross-reactivity or interference between Mouse TMPO and analogues was observed.
Sequence Length
693
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lamina-associated polypeptide 2, isoform alpha (TMPO) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lamina-associated polypeptide 2, isoform alpha (TMPO) Standard Curve.)
Related Product Information for LAP2 elisa kit
Description: This Mouse Lamina-associated polypeptide 2, isoform alpha (TMPO) ELISA Kit employs a two-site sandwich ELISA to quantitate TMPO in samples. An antibody specific for TMPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMPO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPO bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Thymopoietin is a protein involved in the induction of CD90 in the thymus. The thymopoetin (TMPO) gene encodes three alternatively spliced mRNAs encoding proteins of 75 kDa (alpha), 51 kDa (beta) and 39 kDa (gamma) which are ubiquitously expressed in all cells. The human TMPO gene maps to chromosome band 12q22 and consists of eight exons. TMPO alpha is present diffusely expressed with the cell nucleus while TMPO beta and gamma are localized to the nuclear membrane. TMPO beta is a human homolog of the murine protein LAP2 (lamina-associated polypeptide 2). LAP2 plays a role in the regulation of nuclear architecture by binding lamin B1 and chromosomes. This interaction is regulated by phosphorylation during mitosis.
Background: Thymopoietin is a protein involved in the induction of CD90 in the thymus. The thymopoetin (TMPO) gene encodes three alternatively spliced mRNAs encoding proteins of 75 kDa (alpha), 51 kDa (beta) and 39 kDa (gamma) which are ubiquitously expressed in all cells. The human TMPO gene maps to chromosome band 12q22 and consists of eight exons. TMPO alpha is present diffusely expressed with the cell nucleus while TMPO beta and gamma are localized to the nuclear membrane. TMPO beta is a human homolog of the murine protein LAP2 (lamina-associated polypeptide 2). LAP2 plays a role in the regulation of nuclear architecture by binding lamin B1 and chromosomes. This interaction is regulated by phosphorylation during mitosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
lamina-associated polypeptide 2 isoform alpha
NCBI Official Synonym Full Names
thymopoietin
NCBI Official Symbol
Tmpo
NCBI Official Synonym Symbols
TP; LAP2; AI195756; AI606875; AW214352; AW547477; 5630400D24Rik
NCBI Protein Information
lamina-associated polypeptide 2
UniProt Protein Name
Lamina-associated polypeptide 2, isoforms alpha/zeta
Protein Family
UniProt Gene Name
Tmpo
UniProt Synonym Gene Names
Lap2; TP alpha/zeta
UniProt Entry Name
LAP2A_MOUSE
Uniprot Description
TMPO iso6: a single-pass type II membrane protein. Tightly associated with the nuclear lamina. Binds DNA and involved in DNA replication and chromatin remodeling, May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with HAP95. Associated with dilated cardiomyopathy; upregulated in medulloblastoma. Used as a pharmaceutical to treat primary and secondary immune deficiencies, autoimmunity, infections and cancer. Available under the names Timunox (Cilag), Sintomodulina (Italofarmaco) and Mepentil (Recordati). Three alternatively spliced isoforms have been described.
Protein type: Membrane protein, integral; DNA-binding
Cellular Component: nuclear membrane; membrane; integral to membrane; nuclear inner membrane; nuclear envelope; chromosome; chromatin; nucleus
Molecular Function: protein binding; DNA binding
Biological Process: regulation of transcription, DNA-dependent
Research Articles on LAP2
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Product Notes
The Mouse LAP2 tmpo (Catalog #AAA9716762) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716762 ELISA Kit recognizes Mouse LAP2. It is sometimes possible for the material contained within the vial of "Lamina-Associated Polypeptide 2, Isoform alpha (TMPO), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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