Rabbit KCNK2 Polyclonal Antibody | anti-KCNK2 antibody

KCNK2 Antibody

Cat. Num. AAA9612001

• Gene Names: KCNK2; TREK; TPKC1; TREK1; K2p2.1; TREK-1; hTREK-1c; hTREK-1e
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: Peptide affinity purification
• Synonyms: KCNK2; Polyclonal Antibody; KCNK2 Antibody; hTREK 1c; hTREK 1e; K2p2.1; K2P2.1 potassium channel; KCNK 2; Kcnk2; KCNK2_HUMAN; MGC126742; MGC126744; Outward rectifying potassium channel protein TREK 1; Outward rectifying potassium channel protein TREK-1; Outward rectifying potassium channel protein TREK1; Potassium channel subfamily K member 2; Potassium inwardly rectifying channel subfamily K member 2; Tandem pore domain potassium channel TREK 1; Tandem pore domain potassium channel TREK1; TPKC1; TREK 1; TREK 1 K(+) channel subunit; TREK; TREK-1 K(+) channel subunit; TREK1; TWIK related potassium channel 1; Two pore domain potassium channel TREK 1; Two pore domain potassium channel TREK-1; Two pore domain potassium channel TREK1; Two pore potassium channel 1; Two pore potassium channel TPKC1; anti-KCNK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KCNK2 Antibody detects endogenous levels of total KCNK2.
• Purity/Purification: Peptide affinity purification
• Form/Format: Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 422

• Applicable Applications for anti-KCNK2 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA(peptide): 1:20,000-1:40,000; *The optimal dilutions should be determined by the end user.; ; IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight.
• Immunogen: A synthesized peptide derived from human KCNK2, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20°C. Stable for up to 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Human brain, using KCNK2 Antibody. The lane on the left was treated with blocking peptide.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612001 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-KCNK2 antibody

Function: Ion channel that contributes to passive transmembrane potassium transport (PubMed:23169818). Reversibly converts between a voltage-insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner (PubMed:11319556). In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2. In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 (By similarity).Does not display channel activity but reduces the channel activity of isoform 1 and isoform 2 and reduces cell surface expression of isoform 2.

Post Translational Modifications: Phosphorylation at Ser-348 controls the reversible conversion from a leak channel to a voltage-dependent channel.

Subcellular Location: Cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Tissue Specificity: Isoform 4 is detected in kidney, adrenal gland and brain where it is preferentially expressed in the amygdala but not found in thalamus, hypothalamus, hippocampus or substantia nigra.

Subunit Structure: Homodimer; disulfide-linked (Ref.13). Heterodimer with KCNK1; disulfide-linked (By similarity). Interacts with BVES; the interaction enhances KCNK2 surface expression and is inhibited by cAMP (PubMed:26642364).

Similarity: The C-terminal region of isoform 4 mediates its intracellular retention.Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.

NCBI and Uniprot Product Information

• NCBI GI #: 62912487
• NCBI GeneID: 3776
• NCBI Accession #: NP_001017424.1
• NCBI GenBank Nucleotide #: NP_001017424.1
• UniProt Accession #: O95069
• Molecular Weight: 47 kDa
• NCBI Official Full Name: potassium channel subfamily K member 2 isoform a
• NCBI Official Synonym Full Names: potassium two pore domain channel subfamily K member 2
• NCBI Official Symbol: KCNK2
• NCBI Official Synonym Symbols: TREK; TPKC1; TREK1; K2p2.1; TREK-1; hTREK-1c; hTREK-1e
• NCBI Protein Information: potassium channel subfamily K member 2
• UniProt Protein Name: Potassium channel subfamily K member 2
• Protein Family: Potassium channel subfamily
• UniProt Gene Name: KCNK2
• UniProt Synonym Gene Names: TREK; TREK1

NCBI Description

This gene encodes one of the members of the two-pore-domain background potassium channel protein family. This type of potassium channel is formed by two homodimers that create a channel that leaks potassium out of the cell to control resting membrane potential. The channel can be opened, however, by certain anesthetics, membrane stretching, intracellular acidosis, and heat. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Ion channel that contributes to passive transmembrane potassium transport (PubMed:23169818). Reversibly converts between a voltage-insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner (PubMed:11319556). In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2. In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 ().