Rabbit KCNK1 Polyclonal Antibody | anti-KCNK1 antibody

KCNK1 Polyclonal Antibody

Cat. Num. AAA3012947

• Gene Names: KCNK1; DPK; HOHO; K2P1; KCNO1; TWIK1; K2p1.1; TWIK-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide affinity purified.
• Synonyms: KCNK1; Polyclonal Antibody; KCNK1 Polyclonal Antibody; HOHO1; KCNO1; TWIK1; DPK; HOHO; inward rectifying potassium channel protein TWIK 1; Inward rectifying potassium channel protein TWIK-1; Inward rectifying potassium channel protein TWIK1; K2P1; K2p1.1; OTTHUMP00000036029; Potassium channel KCNO1; Potassium channel subfamily K member 1; Potassium inwardly rectifying channel subfamily K member 1; TWIK 1; anti-KCNK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KCNK1 polyclonal antibody detects endogenous levels of KCNK1 protein.
• Purity/Purification: Peptide affinity purified.
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 336

• Applicable Applications for anti-KCNK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Synthetic peptide within human KCNK1 aa 40-100.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of KCNK1 on different cell lysate using anti-KCNK1 antibody at 1/500 dilution. Positive control: Lane1: Human placenta Lane2: Human liver)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-KCNK1 antibody

Ion channel that contributes to passive transmembrane potassium transport and to the regulation of the resting membrane potential in brain astrocytes, but also in kidney and in other tissues. Forms dimeric channels through which potassium ions pass in accordance with their electrochemical gradient. The channel is selective for K+ ions at physiological potassium concentrations and at neutral pH, but becomes permeable to Na+ at subphysiological K+ levels and upon acidification of the extracellular medium. Channel activity is modulated by activation of serotonin receptors. Heterodimeric channels containing KCNK1 and KCNK2 have much higher activity, and may represent the predominant form in astrocytes. Heterodimeric channels containing KCNK1 and KCNK3 or KCNK9 have much higher activity. Heterodimeric channels formed by KCNK1 and KCNK9 may contribute to halothane-sensitive currents. Mediates outward rectifying potassium currents in dentate gyrus granule cells and contributes to the regulation of their resting membrane potential. Contributes to the regulation of action potential firing in dentate gyrus granule cells and down-regulates their intrinsic excitability. In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1.

NCBI and Uniprot Product Information

• NCBI GI #: 4504847
• NCBI GeneID: 3775
• NCBI Accession #: NP_002236.1
• NCBI GenBank Nucleotide #: NP_002236.1
• UniProt Accession #: O00180; O08581; Q9Z2T2
• Molecular Weight: 38kDa
• NCBI Official Full Name: potassium channel subfamily K member 1
• NCBI Official Synonym Full Names: potassium two pore domain channel subfamily K member 1
• NCBI Official Symbol: KCNK1
• NCBI Official Synonym Symbols: DPK; HOHO; K2P1; KCNO1; TWIK1; K2p1.1; TWIK-1
• NCBI Protein Information: potassium channel subfamily K member 1
• UniProt Protein Name: Potassium channel subfamily K member 1
• Protein Family: Potassium channel subfamily
• UniProt Gene Name: KCNK1
• UniProt Synonym Gene Names: HOHO1; KCNO1; TWIK1
• UniProt Entry Name: KCNK1_HUMAN

NCBI Description

This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. [provided by RefSeq, Jul 2008]

Uniprot Description

KCNK1: Weakly inward rectifying potassium channel. Homodimer (Potential). Widely expressed with high levels in heart and brain and lower levels in placenta, lung, liver and kidney. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q42-q43

Cellular Component: voltage-gated potassium channel complex; brush border membrane; apical plasma membrane; plasma membrane; endosome

Molecular Function: inward rectifier potassium channel activity

Biological Process: response to nicotine; synaptic transmission; potassium ion transport