Rabbit ATG4C Polyclonal Antibody | anti-ATG4C antibody

ATG4C Polyclonal Antibody

Cat. Num. AAA3007346

• Gene Names: ATG4C; APG4C; AUTL1; AUTL3; APG4-C
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ATG4C; Polyclonal Antibody; ATG4C Polyclonal Antibody; APG4 autophagy 4 homolog C (S. cerevisiae); APG4 autophagy 4 homolog C; APG4 C; APG4C; ATG 4C; ATG4 autophagy related 4 homolog C (S. cerevisiae); Atg4c; AUT (S. cerevisiae) like 1; cysteine endopeptidase; AUT like 1; cysteine endopeptidase (S. cerevisiae); AUT like 3 cysteine endopeptidase; AUT-like 3 cysteine endopeptidase; AUTL1; AUTL3; Autophagin 3; Autophagin-3; Autophagy related cysteine endopeptidase 3; Autophagy related protein 4 homolog C; anti-ATG4C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATG4C polyclonal antibody detects endogenous levels of ATG4C protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 458

• Applicable Applications for anti-ATG4C antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human ATG4C.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ATG4C pAb at 1:2000 dilutionLane1:U-87MG whole cell lysate (20ug) Lane2:PC3 whole cell lysate (20ug) Lane3:K562 whole cell lysate (40ug) Lane4:The Brain tissue lysate of Rat (10ug) Lane5:AML-12 whole cell lysate (40ug) Lane6:The Muscle tissue lysate of Mouse (40ug))

Related Product Information for anti-ATG4C antibody

Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

NCBI and Uniprot Product Information

• NCBI GI #: 30410844
• NCBI GeneID: 84938
• NCBI Accession #: NP_116241.2
• NCBI GenBank Nucleotide #: NP_116241.2
• UniProt Accession #: Q96DT6
• Molecular Weight: ~ 53kDa
• NCBI Official Full Name: cysteine protease ATG4C
• NCBI Official Synonym Full Names: autophagy related 4C cysteine peptidase
• NCBI Official Symbol: ATG4C
• NCBI Official Synonym Symbols: APG4C; AUTL1; AUTL3; APG4-C
• NCBI Protein Information: cysteine protease ATG4C
• UniProt Protein Name: Cysteine protease ATG4C
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4C
• UniProt Synonym Gene Names: APG4C; AUTL1; AUTL3
• UniProt Entry Name: ATG4C_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4C: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Highly expressed in skeletal muscle, heart, liver and testis. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family.

Protein type: EC 3.4.22.-; Protease; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: cytoplasm; extracellular region; cytosol

Molecular Function: peptidase activity; cysteine-type endopeptidase activity

Biological Process: protein delipidation; mitochondrion degradation; autophagy; protein processing; C-terminal protein lipidation; proteolysis; protein targeting to membrane; autophagic vacuole formation; cellular response to nitrogen starvation