Human TUBG1 (gamma Tubulin) ELISA Kit | TUBG1 elisa kit

Human TUBG1 (gamma Tubulin) ELISA Kit

Cat. Num. AAA7612335

• Gene Names: TUBG1; TUBG; GCP-1; TUBGCP1
• Reactivity: Human
• Synonyms: TUBG1 (gamma Tubulin); Human TUBG1 (gamma Tubulin) ELISA Kit; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1; GCP-1; TUBG; TUBG1; TUBG1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TUBG1. No significant cross-reactivity or interference between TUBG1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TUBG1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 31543831
• NCBI GeneID: 7283
• NCBI Accession #: NP_001061.2
• NCBI GenBank Nucleotide #: NM_001070.4
• UniProt Accession #: P23258; Q53X79; Q9BW59
• Molecular Weight: 51,170 Da
• NCBI Official Full Name: tubulin gamma-1 chain
• NCBI Official Synonym Full Names: tubulin, gamma 1
• NCBI Official Symbol: TUBG1
• NCBI Official Synonym Symbols: TUBG; GCP-1; TUBGCP1
• NCBI Protein Information: tubulin gamma-1 chain; gamma-1-tubulin; tubulin, gamma polypeptide; gamma-tubulin complex component 1
• UniProt Protein Name: Tubulin gamma-1 chain
• UniProt Gene Name: TUBG1
• UniProt Synonym Gene Names: TUBG; GCP-1
• UniProt Entry Name: TBG1_HUMAN

NCBI Description

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]

Uniprot Description

TUBG1: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO/C1orf88. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: centriole; centrosome; recycling endosome; pericentriolar material; condensed nuclear chromosome; cytoplasmic microtubule; nonmotile primary cilium; apical part of cell; cytoplasm; leading edge; polar microtubule; cytosol; gamma-tubulin complex

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: metabolic process; organelle organization and biogenesis; mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; microtubule nucleation; G2/M transition of mitotic cell cycle; cytoplasmic microtubule organization and biogenesis; meiotic spindle organization and biogenesis

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 4