Rabbit anti-Human, Mouse IRS-p53 Polyclonal Antibody | anti-Ikbkb antibody

IRS-p53 (Insulin Receptor Substrate p53, BAIAP2, Brain-enriched scaffolding protein)

Cat. Num. AAA647761

• Gene Names: Ikbkb; IKK2; AIM-1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: IRS-p53; Polyclonal Antibody; IRS-p53 (Insulin Receptor Substrate p53; BAIAP2; Brain-enriched scaffolding protein); Anti -IRS-p53 (Insulin Receptor Substrate p53; anti-Ikbkb antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human interferon beta (IFN-beta), a 22kD member of the type I alpha/beta interferon family. Species Crossreactivity: mouse
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-Ikbkb antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 5ug/ml, detects a band of ~15kD in A20 cell lysates.; Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer, pH 6.0 is recommended.
• Immunogen: Synthetic peptide corresponding to 17aa near the center of human Interferon beta.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Ikbkb antibody

IFN beta, initially described as fibroblast interferon, is produced predominantly by fibroblasts and some epithelial cells. Type 1 interferons are produced primarily in response to viral infections as part of the host immune response and can also inhibit the development of tumors. IFN-beta is involved in the regulation of non-specific humoral immune responses and responses against viral infections.

Product Categories/Family for anti-Ikbkb antibody

Antibodies; Abs to Interferon

NCBI and Uniprot Product Information

• NCBI GI #: 158508716
• NCBI GeneID: 84351
• NCBI Accession #: NP_445807.2
• NCBI GenBank Nucleotide #: NM_053355.2
• UniProt Accession #: Q9QY78
• Molecular Weight: 86,866 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit beta
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• NCBI Official Symbol: Ikbkb
• NCBI Official Synonym Symbols: IKK2; AIM-1
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit beta; IKK-B; NFKBIKB; IKK-beta; I-kappa-B kinase 2; I-kappa-B-kinase beta; inhibitor of kappaB kinase beta; nuclear factor NF-kappa-B inhibitor kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Gene Name: Ikbkb
• UniProt Synonym Gene Names: Ikkb; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
• UniProt Entry Name: IKKB_RAT

NCBI Description

kinase that may be involved in megakaryocyte polyploidization [RGD, Feb 2006]

Uniprot Description

Function: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation

By similarity.

Catalytic activity: ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

Subunit structure: Component of the I-kappa-B-kinase (IKK) canonical complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with TRIM21

By similarity. Interacts with NLRC5; prevents IKBKB phosphorylation and kinase activity

By similarity. Interacts with PDPK1

By similarity. Interacts with EIF2AK2/PKR

By similarity. The phosphorylated form interacts with PPM1A and PPM1B

By similarity. Ref.3

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity. Membrane raft

By similarity. Note: Colocalized with DPP4 in membrane rafts

By similarity.

Domain: The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG

By similarity.

Post-translational modification: Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B

By similarity. Ref.2Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway

By similarity.Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. 'Ser-163' may not serve as a monoubiquitination site. Ubiquitination on 'Ser-163' may modulate phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is disrupted by Yersinia yopJ

By similarity.Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappaB

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.Contains 1 protein kinase domain.